Quality control for Adeno-associated viral vector production.

腺相关病毒载体生产的质量控制。

• 发表时间: 2023
• 来源期刊: Neuromethods
• DOI: 10.1007/978-1-0716-2918-5_5
• PMID: 38585382
• 文章类型: Journal Article
• 作者列表: Ersing I,Rego M,Wang C,Zhang Y,DeMaio KH,Tillgren M,Fava A,Clouse G,Patrick M,Guerin K,Fan M

关键词: AAV; Adeno-associated viral vectors; quality control; rAAV; viral vectors

Abstract

Adeno-associated viral vectors (AAV) are frequently used by neuroscientists to deliver tools, such as biosensors and optogenetic and chemogenetic actuators, in vivo. Despite its widespread use, AAV vector characterization and quality control can vary between labs and viral vector cores leading to variable results and irreproducibility. This protocol describes some of the characterization and quality control assays necessary to confirm an AAV vector\'s titer, genomic identity, serotype and purity.

摘要

神经科学家经常使用腺相关病毒载体（AAV）来提供工具，如生物传感器和光遗传学和化学遗传学执行器，在体内。尽管它广泛使用，AAV载体表征和质量控制可以在实验室和病毒载体核心之间变化,导致可变的结果和不可重复性。该协议描述了一些必要的表征和质量控制测定，以确认AAV载体的滴度，基因组身份，血清型和纯度。