PDF(Pubmed)
Abstract：
UNASSIGNED: To investigate the serum biomarkers in patients with drug-resistant epilepsy (DRE).
UNASSIGNED: A total of 9 DRE patients and 9 controls were enrolled. Serum from DRE patients was prospectively collected and analyzed for potential serum biomarkers using TMT18-labeled proteomics. After fine quality control, bioinformatics analysis was conducted to find differentially expressed proteins. Pathway enrichment analysis identified some biological features shared by differential proteins. Protein-protein interaction (PPI) network analysis was further performed to discover the core proteins.
UNASSIGNED: A total of 117 serum differential proteins were found in our study, of which 44 were revised upwards and 73 downwards. The up-regulated proteins mainly include UGGT2, PDIA4, SEMG1, KIAA1191, CCT7 etc. and the down-regulated proteins mainly include ROR1, NIF3L1, ITIH4, CFP, COL11A2 etc. Pathway enrichment analysis identified that the upregulated proteins were mainly enriched in processes such as immune response, extracellular exosome, serine-type endopeptidase activity and complement and coagulation cascades, and the down-regulated proteins were enriched in signal transduction, extracellular exosome, zinc/calcium ion binding and metabolic pathways. PPI network analysis revealed that the core proteins nodes include PRDX6, CAT, PRDX2, SOD1, PARK7, GSR, TXN, ANXA1, HINT1, and S100A8 etc.
UNASSIGNED: The discovery of these differential proteins enriched our understanding of serum biomarkers in patients with DRE and potentially provides guidance for future targeted therapy.
UNASSIGNED: A total of 9 DRE patients and 9 controls were enrolled. Serum from DRE patients was prospectively collected and analyzed for potential serum biomarkers using TMT18-labeled proteomics. After fine quality control, bioinformatics analysis was conducted to find differentially expressed proteins. Pathway enrichment analysis identified some biological features shared by differential proteins. Protein-protein interaction (PPI) network analysis was further performed to discover the core proteins.
UNASSIGNED: A total of 117 serum differential proteins were found in our study, of which 44 were revised upwards and 73 downwards. The up-regulated proteins mainly include UGGT2, PDIA4, SEMG1, KIAA1191, CCT7 etc. and the down-regulated proteins mainly include ROR1, NIF3L1, ITIH4, CFP, COL11A2 etc. Pathway enrichment analysis identified that the upregulated proteins were mainly enriched in processes such as immune response, extracellular exosome, serine-type endopeptidase activity and complement and coagulation cascades, and the down-regulated proteins were enriched in signal transduction, extracellular exosome, zinc/calcium ion binding and metabolic pathways. PPI network analysis revealed that the core proteins nodes include PRDX6, CAT, PRDX2, SOD1, PARK7, GSR, TXN, ANXA1, HINT1, and S100A8 etc.
UNASSIGNED: The discovery of these differential proteins enriched our understanding of serum biomarkers in patients with DRE and potentially provides guidance for future targeted therapy.
摘要：
■研究耐药癫痫（DRE）患者的血清生物标志物。
■共纳入9名DRE患者和9名对照。前瞻性收集DRE患者的血清，并使用TMT18标记的蛋白质组学分析潜在的血清生物标志物。经过精细的质量控制，进行生物信息学分析以找到差异表达的蛋白质。途径富集分析确定了差异蛋白共有的一些生物学特征。进一步进行蛋白质-蛋白质相互作用（PPI）网络分析以发现核心蛋白。
■在我们的研究中总共发现了117种血清差异蛋白，其中44项向上修订，73项向下修订。上调蛋白主要包括UGGT2、PDIA4、SEMG1、KIAA1191、CCT7等。下调蛋白主要包括ROR1、NIF3L1、ITIH4、CFP、COL11A2等.通路富集分析确定上调蛋白主要富集在免疫应答等过程中,细胞外外泌体，丝氨酸型内肽酶活性和补体和凝血级联反应，下调的蛋白质在信号转导中富集，细胞外外泌体，锌/钙离子结合和代谢途径。PPI网络分析显示，核心蛋白节点包括PRDX6、CAT、PRDX2,SOD1,PARK7,GSR,TXN,ANXA1、HINT1和S100A8等.
■这些差异蛋白的发现丰富了我们对DRE患者血清生物标志物的理解，并可能为未来的靶向治疗提供指导。
■共纳入9名DRE患者和9名对照。前瞻性收集DRE患者的血清，并使用TMT18标记的蛋白质组学分析潜在的血清生物标志物。经过精细的质量控制，进行生物信息学分析以找到差异表达的蛋白质。途径富集分析确定了差异蛋白共有的一些生物学特征。进一步进行蛋白质-蛋白质相互作用（PPI）网络分析以发现核心蛋白。
■在我们的研究中总共发现了117种血清差异蛋白，其中44项向上修订，73项向下修订。上调蛋白主要包括UGGT2、PDIA4、SEMG1、KIAA1191、CCT7等。下调蛋白主要包括ROR1、NIF3L1、ITIH4、CFP、COL11A2等.通路富集分析确定上调蛋白主要富集在免疫应答等过程中,细胞外外泌体，丝氨酸型内肽酶活性和补体和凝血级联反应，下调的蛋白质在信号转导中富集，细胞外外泌体，锌/钙离子结合和代谢途径。PPI网络分析显示，核心蛋白节点包括PRDX6、CAT、PRDX2,SOD1,PARK7,GSR,TXN,ANXA1、HINT1和S100A8等.
■这些差异蛋白的发现丰富了我们对DRE患者血清生物标志物的理解，并可能为未来的靶向治疗提供指导。
