PDF(Pubmed)
Abstract：
Proteomic profiling on other primary cicatricial alopecias, such as frontal fibrosing alopecia and lichen planopilaris, have suggested a T helper 1-mediated inflammatory pathway, but in central centrifugal cicatricial alopecia (CCCA), the protein expression patterns are unknown. In this study, we sought to characterize protein expression patterns in CCCA to identify biomarkers of disease activity that will identify potential therapeutic avenues for treatment. Scalp protein quantification was performed to understand protein expression patterns in affected versus unaffected scalps in CCCA. A total of 5444 proteins were identified, of which 148 proteins were found to be differentially expressed in CCCA-affected scalp, with upregulation of adaptive immune pathways (IGHG3, P = .034; IGHG4, P = .01; IGG1, P = .026) and markers of fibrosis (ITGA1, P = .016; SFRP2, P = .045; TPM2, P = .029; SLMAP, P = .016) and downregulation of metabolic proteins (ALOX15B, P = .003; FADS2, P = .006; ELOVL5, P = .007; FA2H, P = .017; FAR2, P = .011; SC5D, P < .001). Our analysis revealed, to our knowledge, previously unknown humoral immune canonical pathways, notably IgG, implicated in CCCA and additionally confirmed aberrant lipid metabolism pathways implicated in diabetes mellitus, suggesting unique mechanisms of disease in patients with CCCA.
摘要：
其他原发性瘢痕性脱发的蛋白质组学分析，如额叶纤维化脱发和扁平苔藓，已经提出了T辅助1介导的炎症途径，但在中央离心瘢痕性脱发（CCCA），蛋白质表达模式未知。在这项研究中,我们试图表征CCCA中的蛋白质表达模式,以鉴定疾病活动的生物标志物,从而确定潜在的治疗途径.进行头皮蛋白定量以了解CCCA中受影响的头皮与未受影响的头皮中的蛋白表达模式。总共鉴定了5444种蛋白质，其中148种蛋白质在受CCCA影响的头皮中差异表达，随着适应性免疫途径的上调（IGHG3，P=.034；IGHG4，P=.01；IGG1，P=.026）和纤维化标志物（ITGA1，P=.016；SFRP2，P=.045；TPM2，P=.029；SLMAP，P=.016）和代谢蛋白的下调（ALOX15B，P=.003;FADS2,P=.006;ELOVL5,P=.007;FA2H,P=.017；FAR2，P=.011；SC5D，P<.001)。我们的分析显示，根据我们的知识，以前未知的体液免疫规范途径，特别是IgG，与CCCA有关，并进一步证实了与糖尿病有关的异常脂质代谢途径，提示CCCA患者独特的疾病机制。
