Abstract：
Myzus persicae is an important pest that has developed resistance to nearly all currently used insecticidal products. The employment of insecticide synergists is one of the effective strategies that need to be developed for the management of this resistance. Our study showed that treatment with a combination of the antibiotic, rifampicin, with imidacloprid, cyantraniliprole, or clothianidin significantly increased their toxicities against M. persicae, by 2.72, 3.59, and 2.41 folds, respectively. Rifampicin treatment led to a noteworthy reduction in the activities of multifunctional oxidases (by 32.64%) and esterases (by 23.80%), along with a decrease in the expression of the CYP6CY3 gene (by 58.57%) in M. persicae. It also negatively impacted the fitness of the aphids, including weight, life span, number of offspring, and elongation of developmental duration. In addition, bioassays showed that the combination of rifampicin and a detoxification enzyme inhibitor, piperonyl butoxide, or dsRNA of CYP6CY3 further significantly improved the toxicity of imidacloprid against M. persicae, by 6.19- and 7.55-fold, respectively. The present study suggests that development of active ingredients such as rifampicin as candidate synergists, show promise to overcome metabolic resistance to insecticides in aphids.
摘要：
桃树是一种重要的害虫，对几乎所有目前使用的杀虫产品都产生了抗性。使用杀虫剂增效剂是管理这种抗性需要开发的有效策略之一。我们的研究表明，用抗生素联合治疗，利福平,与吡虫啉，cyantraniliprole,或clothianidin显着增加了它们对persicae的毒性，2.72、3.59和2.41倍，分别。利福平处理导致多功能氧化酶（32.64％）和酯酶（23.80％）的活性显着降低，随着CYP6CY3基因的表达减少（减少58.57％）。这也对蚜虫的健康产生了负面影响，包括体重,寿命,后代的数量，和发育持续时间的延长。此外,生物测定表明，利福平和解毒酶抑制剂的组合，胡椒基丁醚，或CYP6CY3的dsRNA进一步显着提高了吡虫啉对桃丝的毒性，由6.19倍和7.55倍，分别。本研究表明，开发活性成分如利福平作为候选增效剂，有望克服蚜虫对杀虫剂的代谢抗性。
