PDF(Pubmed)
Abstract：
BACKGROUND: The gut microbiota, vital for host health, influences metabolism, immune function, and development. Understanding the dynamic processes of bacterial accumulation within the gut is crucial, as it is closely related to immune responses, antibiotic resistance, and colorectal cancer. We investigated Escherichia coli behavior and distribution in zebrafish larval intestines, focusing on the gut microenvironment.
RESULTS: We discovered that E. coli spread was considerably suppressed within the intestinal folds, leading to a strong physical accumulation in the folds. Moreover, a higher concentration of E. coli on the dorsal side than on the ventral side was observed. Our in vitro microfluidic experiments and theoretical analysis revealed that the overall distribution of E. coli in the intestines was established by a combination of physical factor and bacterial taxis.
CONCLUSIONS: Our findings provide valuable insight into how the intestinal microenvironment affects bacterial motility and accumulation, enhancing our understanding of the behavioral and ecological dynamics of the intestinal microbiota.
RESULTS: We discovered that E. coli spread was considerably suppressed within the intestinal folds, leading to a strong physical accumulation in the folds. Moreover, a higher concentration of E. coli on the dorsal side than on the ventral side was observed. Our in vitro microfluidic experiments and theoretical analysis revealed that the overall distribution of E. coli in the intestines was established by a combination of physical factor and bacterial taxis.
CONCLUSIONS: Our findings provide valuable insight into how the intestinal microenvironment affects bacterial motility and accumulation, enhancing our understanding of the behavioral and ecological dynamics of the intestinal microbiota.
摘要：
背景：肠道菌群，对宿主健康至关重要，影响新陈代谢，免疫功能，和发展。了解肠道内细菌积累的动态过程至关重要，因为它与免疫反应密切相关，抗生素耐药性,还有结直肠癌.我们调查了大肠杆菌在斑马鱼幼虫肠道中的行为和分布，关注肠道微环境。
结果：我们发现大肠杆菌在肠道褶皱中的传播受到了很大的抑制，导致褶皱中强烈的物理积累。此外，观察到背侧的大肠杆菌浓度高于腹侧。我们的体外微流控实验和理论分析表明，大肠杆菌在肠道中的总体分布是通过物理因素和细菌出租车的组合来确定的。
结论：我们的研究结果为肠道微环境如何影响细菌运动和积累提供了有价值的见解，增强我们对肠道微生物群的行为和生态动力学的理解。
结果：我们发现大肠杆菌在肠道褶皱中的传播受到了很大的抑制，导致褶皱中强烈的物理积累。此外，观察到背侧的大肠杆菌浓度高于腹侧。我们的体外微流控实验和理论分析表明，大肠杆菌在肠道中的总体分布是通过物理因素和细菌出租车的组合来确定的。
结论：我们的研究结果为肠道微环境如何影响细菌运动和积累提供了有价值的见解，增强我们对肠道微生物群的行为和生态动力学的理解。
