Abstract：
Nucleic acid detection is conducive to preventing the spread of COVID-19 pandemic. In this work, we successfully designed a soft interface confined DNA walker by anchoring hairpin reporter probes on cell membranes for the detection of SARS-CoV-2 variants. In the presence of target RNA, the cyclic self-assembly reaction occurred between hairpin probes H1 and H2, and the continuous walking of target RNA on cell membranes led to the gradual amplification of fluorescence signal. The enrichment of H1 on membranes and the unique fluidity of membranes promoted the collision efficiency between DNA strands in the reaction process, endowing this method with high sensitivity. In addition, the double-blind test of synthetic RNA in 5% normal human serum demonstrated the good stability and anti-interference in complex environment of this method, which exhibited great potential in clinical diagnostics.
摘要：
核酸检测有利于防止COVID-19大流行的传播。在这项工作中,我们通过将发夹报告探针锚定在细胞膜上，成功地设计了一种软界面限制的DNA步行器，用于检测SARS-CoV-2变体。在靶RNA的存在下，发夹探针H1和H2之间发生循环自组装反应，目标RNA在细胞膜上的连续行走导致荧光信号逐渐放大。H1在膜上的富集和膜独特的流动性促进了反应过程中DNA链之间的碰撞效率，该方法具有较高的灵敏度。此外,在5%正常人血清中合成RNA的双盲试验表明,该方法具有良好的稳定性和在复杂环境下的抗干扰性,在临床诊断中表现出巨大的潜力。
