Abstract：
In this chapter, we provide detailed instructions to perform quantitative reflectance imaging in a mouse model of a rare epidermal disorder caused by hyperactive connexin 26 hemichannels. Reflectance imaging is a versatile and powerful tool in dermatology, offering noninvasive, high-resolution insights into skin pathology, which is essential for both clinical practice and research. This approach offers several advantages and applications. Unlike traditional biopsy, reflectance imaging is noninvasive, allowing for real-time, in vivo examination of the skin. This is particularly valuable for monitoring chronic conditions or assessing the efficacy of treatments over time, enabling the detailed examination of skin morphology. This is crucial for identifying features of skin diseases such as cancers, inflammatory conditions, and infections. In therapeutic applications, reflectance imaging can be used to monitor the response of skin lesions to treatments. It can help in identifying the most representative area of a lesion for biopsy, thereby increasing the diagnostic accuracy. Reflectance imaging can also be used to diagnose and monitor inflammatory skin diseases, like psoriasis and eczema, by visualizing changes in skin structure and cellular infiltration. As the technology becomes more accessible, it has potential in telemedicine, allowing for remote diagnosis and monitoring of skin conditions. In academic settings, reflectance imaging can be a powerful research tool, enabling the study of skin pathology and the effects of novel treatments, including the development of monoclonal antibodies for therapeutic applications.
摘要：
在这一章中,我们提供了详细的说明，以在由过度活跃的连接蛋白26半通道引起的罕见表皮疾病的小鼠模型中进行定量反射成像。反射成像是皮肤病学中的多功能和强大的工具，提供非侵入性，对皮肤病理学的高分辨率见解，这对临床实践和研究都至关重要。这种方法提供了几个优点和应用。与传统的活检不同,反射成像是非侵入性的，允许实时，体内皮肤检查。这对于监测慢性病或评估治疗随着时间的推移的疗效特别有价值。使皮肤形态的详细检查。这对于识别癌症等皮肤病的特征至关重要，炎症条件，和感染。在治疗应用中，反射成像可用于监测皮肤损伤对治疗的反应。它可以帮助识别病变的最具代表性的区域进行活检，从而提高诊断的准确性。反射成像也可用于诊断和监测炎症性皮肤病，比如牛皮癣和湿疹，通过可视化皮肤结构和细胞浸润的变化。随着技术的普及，它在远程医疗方面有潜力，允许远程诊断和监测皮肤状况。在学术环境中，反射成像可以成为一个强大的研究工具，能够研究皮肤病理学和新疗法的效果，包括用于治疗应用的单克隆抗体的开发。
