Abstract：
Connexins (Cxs) are transmembrane proteins which form hemichannels and gap junction channels at the plasma membrane. These channels allow the exchange of ions and molecules between the intra- and extracellular space and between cytoplasm of adjacent cells, respectively. The channel function of Cx assemblies has been extensively studied; however, \"noncanonical\" functions have emerged in the last few decades and have capture the attentions of many researchers, including the role of some Cxs as gene modulators or transcription factors. In this chapter, we describe a protocol to study the interaction of Cx46 with DNA in HeLa cells. These methods can facilitate understanding the role of Cxs in physiological processes and pathological mechanisms, including, for example, the contribution of Cx46 in maintaining stemness of glioma cancer stem cells.
摘要：
连接蛋白(Cxs)是跨膜蛋白,其在质膜处形成半通道和间隙连接通道。这些通道允许在细胞内和细胞外空间之间以及相邻细胞的细胞质之间交换离子和分子。分别。Cx组件的通道功能已被广泛研究；然而，“非规范函数”在过去的几十年中出现，并引起了许多研究人员的关注，包括一些Cxs作为基因调节剂或转录因子的作用。在这一章中,我们描述了研究Cx46与HeLa细胞中DNA相互作用的方案。这些方法有助于理解Cxs在生理过程和病理机制中的作用。包括,例如,Cx46在维持神经胶质瘤肿瘤干细胞干性方面的贡献。
