PDF(Pubmed)
Abstract：
The diagnosis of Q fever is challenging due to nonspecific symptoms and negative standard blood culture results. Serological testing through immunofluorescence assay (IFA) is the most commonly used method for diagnosing this disease. Polymerase chain reaction (PCR) tests can also be used to detect bacterial DNA if taken at an appropriate time. Once the presence of bacteria is confirmed in a sample, an enrichment step is required before characterizing it through sequencing. Cultivating C. burnetii is challenging as it can only be isolated by inoculation into cell culture, embryonated eggs, or animals. In this article, we describe the isolation of C. burnetii from a valve specimen in Vero cells. We conducted genome sequencing and taxonomy profiling of this isolate and were able to determine its taxonomic affiliation. Furthermore, Multispacer sequence typing (MST) analysis suggests that the infection originated from a local strain of C. burnetii found around northern Israel and Lebanon. This novel strain belongs to a previously described genotype MST6, harboring the QpRS plasmid, never reported in Israel.
摘要：
由于非特异性症状和阴性标准血培养结果，Q热的诊断具有挑战性。通过免疫荧光测定（IFA）进行的血清学测试是诊断该疾病的最常用方法。如果在适当的时间进行聚合酶链反应（PCR）测试，也可以用于检测细菌DNA。一旦确认样本中存在细菌，在通过测序表征之前需要富集步骤。培养C.burnetii具有挑战性，因为它只能通过接种到细胞培养物中进行分离。胚胎蛋,或动物。在这篇文章中,我们描述了在Vero细胞中从瓣膜样本中分离伯氏梭菌。我们对该分离物进行了基因组测序和分类学分析，并能够确定其分类学隶属关系。此外，多间隔区序列分型（MST）分析表明，感染起源于在以色列北部和黎巴嫩北部发现的当地C.burnetii菌株。这种新型菌株属于先前描述的基因型MST6，带有QpRS质粒，从未在以色列报道过。
