METHODS: A total of 1032 children were recruited, 684 of whom had diarrhea and 348 without, from Yuying Children\'s Hospital in Wenzhou, China. Samples of stool were collected from each participant, followed by extraction of DNA, genotyping, and molecular identification of Cryptosporidium species and subtypes.
RESULTS: Twenty-two of the 1032 (2.1%) children were infected with Cryptosporidium spp. with 2.5% (17/684) and 1.4% (5/348) in diarrhoeic and asymptomatic children, respectively. Four Cryptosporidium species were identified, including C. parvum (68.2%; 15/22), C. felis (13.6%; 3/22), C. viatorum (9.1%; 2/22), and C. baileyi (9.1%; 2/22). Two C. parvum subtypes named IIdA19G1 (n = 14) and IInA10 (n = 1), and one each of C. felis (XIXa) and C. viatorum (XVaA3g) subtype was found as well.
CONCLUSIONS: This is the first research that identified Cryptosporidium in children of Wenzhou, China, using PCR. Identification of zoonotic C. parvum, C. felis, C. viatorum, and their subtypes indicate potential cross-species transmission of Cryptosporidium between children and animals. Additionally, the presence of C. baileyi in children suggests that this species has a wider host range than previously believed and that it possesses the capacity to infect humans.
方法:共招募1032名儿童,其中684人腹泻,348人没有腹泻,温州育英儿童医院,中国。从每个参与者收集粪便样本,然后提取DNA,基因分型,以及隐孢子虫种类和亚型的分子鉴定。
结果:1032名(2.1%)儿童中有22名感染了隐孢子虫。腹泻和无症状儿童占2.5%(17/684)和1.4%(5/348),分别。确定了四种隐孢子虫,包括C.parvum(68.2%;15/22),C.felis(13.6%;3/22),C.viatorum(9.1%;2/22),和C.baileyi(9.1%;2/22)。两种C.parvum亚型命名为IIdA19G1(n=14)和IInA10(n=1),还发现了一种C.felis(XIXa)和C.viatorum(XVaA3g)亚型。
结论:这是首次在温州儿童中发现隐孢子虫的研究,中国,使用PCR。人畜共患parvum的鉴定,C.Felis,C.viatorum,它们的亚型表明隐孢子虫在儿童和动物之间潜在的跨物种传播。此外,儿童中C.baileyi的存在表明,该物种的宿主范围比以前认为的要大,并且具有感染人类的能力。