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Abstract：
Vitronectin (VN) is an extracellular matrix protein with a crucial role in regulating bone remodeling. In this study, we aimed to investigate the effect of VN deficiency in a mouse model of osteoporosis induced by ovariectomy (OVX). The findings revealed that the absence of VN led to an increase in the activity of tartrate-resistant acid phosphatase (TRAP), a marker for osteoclasts, in the plasma of OVX-operated mice. TRAP staining further demonstrated that VN deficiency resulted in a higher number of osteoclasts within the femurs of OVX-operated mice. X-ray micro-computed tomography analysis of the femurs in OVX-operated mice indicated that VN deficiency significantly suppressed the OVX-induced increase of marrow area and total volume of bone. Additionally, we assessed structural model index (SMI) and degree of anisotropy (DA) as indices of osteoporosis. The results showed that VN deficiency effectively attenuated the OVX-induced increase in SMI and DA among OVX-operated mice. In summary, our study demonstrates the vital role of VN in regulating osteoclastogenesis and bone remodeling in the mouse model of osteoporosis.
摘要：
玻连蛋白（VN）是一种细胞外基质蛋白，在调节骨重塑中起着至关重要的作用。在这项研究中,我们的目的是研究VN缺乏在卵巢切除术（OVX）诱导的骨质疏松症小鼠模型中的作用。研究结果表明，VN的缺乏导致抗酒石酸酸性磷酸酶（TRAP）的活性增加，破骨细胞的标记,在OVX手术小鼠的血浆中。TRAP染色进一步证明VN缺乏导致OVX手术小鼠股骨内破骨细胞数量增加。对OVX手术小鼠股骨的X射线显微计算机断层扫描分析表明，VN缺乏症可显着抑制OVX引起的骨髓面积和骨总体积的增加。此外,我们评估了结构模型指数（SMI）和各向异性程度（DA）作为骨质疏松症的指标。结果表明，VN缺乏可有效减轻OVX手术小鼠中OVX诱导的SMI和DA增加。总之,我们的研究表明，在骨质疏松小鼠模型中，VN在调节破骨细胞生成和骨重建中的重要作用。
