Abstract：
Xylitol is an increasingly popular functional food additive, and the newly isolated yeast Wickerhamomyces anomalus WA has shown extensive substrate utilization capability, with the ability to grow on hexose (d-galactose, d-glucose, d-mannose, l-fructose, and d-sorbose) and pentose (d-xylose and l-arabinose) substrates, as well as high tolerance to xylose at concentrations of up to 300 g/L. Optimal xylitol fermentation conditions were achieved at 32 °C, 140 rpm, pH 5.0, and initial cell concentration OD600 of 2.0, with YP (yeast extract 10 g/L, peptone 20 g/L) as the optimal nitrogen source. Xylitol yield increased from 0.61 g/g to 0.91 g/g with an increase in initial substrate concentration from 20 g/L to 180 g/L. Additionally, 20 g/L glycerol was found to be the optimal co-substrate for xylitol fermentation, resulting in an increase in xylitol yield from 0.82 g/g to 0.94 g/g at 140 rpm, enabling complete conversion of xylose to xylitol.
摘要：
木糖醇是一种越来越受欢迎的功能性食品添加剂，新分离的酵母Wickerhamomyces异常WA显示出广泛的底物利用能力，具有在己糖（d-半乳糖，d-葡萄糖，d-甘露糖,l-果糖,和d-山梨糖）和戊糖（d-木糖和l-阿拉伯糖）底物，以及在高达300g/L的浓度下对木糖的高耐受性。在32°C下实现了最佳的木糖醇发酵条件，140转/分,pH5.0，初始细胞浓度OD600为2.0，用YP（酵母提取物10g/L，蛋白胨20g/L)为最佳氮源。木糖醇产量从0.61g/g增加到0.91g/g，初始底物浓度从20g/L增加到180g/L。此外,发现20g/L甘油是木糖醇发酵的最佳共底物，导致木糖醇产量在140rpm下从0.82g/g增加到0.94g/g，使木糖完全转化为木糖醇。
