Abstract：
Four undescribed compounds including three harzianic acids (1, 3 and 4) and one oxazolidinone (2), along with three known ones (5-7) were isolated from the solid fermented product of endophytic fungus Ilyonectria sp., their structures were elucidated as 1-amino-harzianic acid (1), ilyonectria-oxazolidinone (2),10\'-nor- isoharzianic acid (3), isohomoharzianic acid (4), harzianic acid (5), isoharzianic acid (6), homoharzianic acid (7) by means of detailed chemical evidences and spectroscopic data analysis. All the compounds were evaluated for cytotoxicity against SMMC-7721 human cancer cell lines by MTS assay. Among the seven tested compounds, 1-amino-harzianic acid (1) demonstrated well cytotoxic activity against SMMC-7721 with IC50 value of 26.84 μM. The results of molecular docking indicated that compound exhibited moderate anti-tumor activity may through binding to apoptosis related proteins.
摘要：
四种未描述的化合物，包括三种哈齐酸（1、3和4）和一种恶唑烷酮（2），从内生真菌Ilyonectriasp的固体发酵产物中分离出三个已知的（5-7）。,它们的结构被阐明为1-氨基-哈齐酸（1），恶唑烷酮(2)，10'-正-异哈齐酸（3），异高哈齐酸(4)，哈齐酸（5），异harzianacid(6),高哈齐酸（7）通过详细的化学证据和光谱数据分析。通过MTS测定评价所有化合物对SMMC-7721人癌细胞系的细胞毒性。在七个测试化合物中，1-氨基-哈齐酸(1)对SMMC-7721表现出良好的细胞毒性活性,IC50值为26.84μM。分子对接结果表明，化合物可能通过与凋亡相关蛋白结合而表现出中等程度的抗肿瘤活性。
