Abstract：
Cotton fiber holds immense importance as the primary raw material for the textile industry. Consequently, comprehending the regulatory mechanisms governing fiber development is pivotal for enhancing fiber quality. Our study aimed to construct a regulatory network of competing endogenous RNAs (ceRNAs) and assess the impact of non-coding RNAs on gene expression throughout fiber development. Through whole transcriptome data analysis, we identified differentially expressed genes (DEGs) regulated by non-coding RNA (ncRNA) that were predominantly enriched in phenylpropanoid biosynthesis and the fatty acid elongation pathway. This analysis involved two contrasting phenotypic materials (J02-508 and ZRI015) at five stages of fiber development. Additionally, we conducted a detailed analysis of genes involved in fatty acid elongation, including KCS, KCR, HACD, ECR, and ACOT, to unveil the factors contributing to the variation in fatty acid elongation between J02-508 and ZRI015. Through the integration of histochemical GUS staining, dual luciferase assay experiments, and correlation analysis of expression levels during fiber development stages for lncRNA MSTRG.44818.23 (MST23) and GhKCR2, we elucidated that MST23 positively regulates GhKCR2 expression in the fatty acid elongation pathway. This identification provides valuable insights into the molecular mechanisms underlying fiber development, emphasizing the intricate interplay between non-coding RNAs and protein-coding genes.
摘要：
棉纤维作为纺织工业的主要原料具有极其重要的意义。因此,理解控制纤维发育的调节机制对于提高纤维质量至关重要。我们的研究旨在构建竞争性内源性RNA（ceRNA）的调控网络，并评估非编码RNA对整个纤维发育过程中基因表达的影响。通过整个转录组数据分析，我们鉴定了由非编码RNA(ncRNA)调控的差异表达基因(DEGs),这些基因主要富集在类苯丙烷生物合成和脂肪酸延伸途径中.该分析涉及纤维发育的五个阶段的两种对比表型材料(J02-508和ZRI015)。此外,我们对参与脂肪酸延伸的基因进行了详细分析，包括KCS,KCR,HACD,ECR,ACOT,揭示J02-508和ZRI015之间脂肪酸伸长变化的因素。通过组织化学GUS染色的整合，双荧光素酶测定实验，纤维发育阶段lncRNAMSTRG.44818.23（MST23）和GhKCR2的表达水平的相关性分析，我们阐明了MST23在脂肪酸延伸途径中正向调节GhKCR2的表达。这种鉴定为纤维发育的分子机制提供了有价值的见解，强调非编码RNA和蛋白质编码基因之间复杂的相互作用。
