Abstract：
BACKGROUND: Omadacycline is the first aminomethyl-tetracycline variety to successfully enter clinical applications. To support regular therapeutic drug monitoring (TDM) in clinical practice, an ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) method was developed that would allow omadacycline quantification in human serum.
METHODS: Proteins were precipitated from serum samples using methanol. Tigecycline was used as the internal standard. Mobile phase A was formic acid in water (0.1% v/v) and mobile phase B was methanol. UPLC-MS/MS was performed for analyte separation using a gradient elution program at a flow rate of 0.3 mL/min and a total run time of 5 min. The chromatography column was a ZORBAX PRHD SB-Aq (3 × 50 mm, 1.8 μm, Agilent, USA). The multiple reaction monitoring transitions at m/z = 557.4/470.3 and 586.5/513.3 were selected for omadacycline and tigecycline in the positive mode, respectively.
RESULTS: The validated curve ranges were 0.5-25.0 μg/mL. This method exhibited acceptable selectivity, matrix effects, and recovery. The inter- and intra-run accuracies ranged from 93.5% to 114.8%, and the inter- and intra-run precisions were between 1.29% and 5.55%.
CONCLUSIONS: The LC-MS/MS method provided a simple, specific, and rapid quantification of omadacycline in the serum of patients with pulmonary infection.
METHODS: Proteins were precipitated from serum samples using methanol. Tigecycline was used as the internal standard. Mobile phase A was formic acid in water (0.1% v/v) and mobile phase B was methanol. UPLC-MS/MS was performed for analyte separation using a gradient elution program at a flow rate of 0.3 mL/min and a total run time of 5 min. The chromatography column was a ZORBAX PRHD SB-Aq (3 × 50 mm, 1.8 μm, Agilent, USA). The multiple reaction monitoring transitions at m/z = 557.4/470.3 and 586.5/513.3 were selected for omadacycline and tigecycline in the positive mode, respectively.
RESULTS: The validated curve ranges were 0.5-25.0 μg/mL. This method exhibited acceptable selectivity, matrix effects, and recovery. The inter- and intra-run accuracies ranged from 93.5% to 114.8%, and the inter- and intra-run precisions were between 1.29% and 5.55%.
CONCLUSIONS: The LC-MS/MS method provided a simple, specific, and rapid quantification of omadacycline in the serum of patients with pulmonary infection.
摘要：
背景：Omadacycline是第一个成功进入临床应用的氨基甲基-四环素品种。为了支持临床实践中的定期治疗药物监测（TDM），开发了一种超高效液相色谱-串联质谱（UPLC-MS/MS）方法，该方法可以对人血清中的omadacycline进行定量。
方法:使用甲醇从血清样品中沉淀蛋白质。替加环素用作内标。流动相A是甲酸的水溶液(0.1%v/v),流动相B是甲醇。使用梯度洗脱程序以0.3mL/min的流速和5min的总运行时间进行UPLC-MS/MS用于分析物分离。色谱柱为ZORBAXPRHDSB-Aq(3×50mm，1.8μm,安捷伦,美国）。在m/z=557.4/470.3和586.5/513.3处的多反应监测转变被选择用于奥马环素和替加环素的阳性模式,分别。
结果：验证的曲线范围为0.5-25.0μg/mL。该方法表现出可接受的选择性，矩阵效应，和恢复。运行间和运行中的准确度从93.5%到114.8%不等，运行间和运行内精度在1.29%至5.55%之间。
结论：LC-MS/MS方法提供了一种简单的，具体,并快速定量肺部感染患者血清中的奥马环素。
方法:使用甲醇从血清样品中沉淀蛋白质。替加环素用作内标。流动相A是甲酸的水溶液(0.1%v/v),流动相B是甲醇。使用梯度洗脱程序以0.3mL/min的流速和5min的总运行时间进行UPLC-MS/MS用于分析物分离。色谱柱为ZORBAXPRHDSB-Aq(3×50mm，1.8μm,安捷伦,美国）。在m/z=557.4/470.3和586.5/513.3处的多反应监测转变被选择用于奥马环素和替加环素的阳性模式,分别。
结果：验证的曲线范围为0.5-25.0μg/mL。该方法表现出可接受的选择性，矩阵效应，和恢复。运行间和运行中的准确度从93.5%到114.8%不等，运行间和运行内精度在1.29%至5.55%之间。
结论：LC-MS/MS方法提供了一种简单的，具体,并快速定量肺部感染患者血清中的奥马环素。
