Abstract：
Total adenosine triphosphate (tATP) was investigated for its potential as a rapid indicator of cyanobacterial growth and algaecide effectiveness. tATP and other common bloom monitoring parameters were measured over the growth cycles of cyanobacteria and green algae in laboratory cultures and examined at a drinking water source during an active bloom. Strong correlations (R2>0.78) were observed between tATP and chlorophyll-a in cyanobacteria cultures. tATP offered greater sensitivity by increasing two orders of magnitude approximately 7 d before changes in chlorophyll-a or optical density were observed in Lyngbya sp. and Dolichospermum sp. cultures. Increases in tATP per cell coincided with the onset of exponential growth phases in lab cultures and increase in cell abundance in field samples, suggesting that ATP/cell is a sensitive indicator that may be used to identify the development of blooms. Bench-scale trials using samples harvested during a bloom showed that tATP exhibited a clear dose-response during copper sulfate (CuSO4) and hydrogen peroxide (H2O2) treatment compared to chlorophyll-a and cell counts, indicating that cellular production and storage of ATP decreases even when live and dead cells cannot be distinguished. During Copper (Cu) algaecide application at a reservoir used as a drinking water source, tATP and cell counts decreased following initial algaecide application; however, the bloom rebounded within 10 d showing that the Cu algaecide only has limited effectiveness. In this case, tATP was a sensitive indicator to bloom rebounding after algaecide treatments and correlated positively with cell counts (R2=0.7). These results support the use of tATP as a valuable complementary bloom monitoring tool for drinking water utilities to implement during the monitoring and treatment of cyanobacterial blooms.
摘要：
研究了总三磷酸腺苷（tATP）作为蓝细菌生长和除藻效果的快速指标的潜力。在实验室培养中，在蓝细菌和绿藻的生长周期内测量了tATP和其他常见的水华监测参数，并在活跃的水华期间在饮用水源处进行了检查。在蓝细菌培养物中，tATP和叶绿素a之间观察到了很强的相关性（R2>0.78）。tATP通过在Lyngbyasp中观察到叶绿素a或光密度变化之前约7d增加两个数量级，从而提供了更高的灵敏度。和Dolichospermumsp.文化。每个细胞tATP的增加与实验室培养中指数生长期的开始以及野外样品中细胞丰度的增加相吻合。表明ATP/细胞是一个敏感的指标，可用于识别水华的发展。使用开花期间收集的样品进行的基准试验表明，与叶绿素a和细胞计数相比，tATP在硫酸铜（CuSO4）和过氧化氢（H2O2）处理期间表现出明显的剂量反应，表明即使无法区分活细胞和死细胞，ATP的细胞产生和储存也会减少。在用作饮用水源的水库中使用铜（Cu）除藻剂期间，tATP和细胞计数在最初施用除藻剂后减少；然而，水华在10d内反弹，表明铜除藻剂的有效性有限。在这种情况下,tATP是除藻后水华反弹的敏感指标，与细胞计数呈正相关（R2=0.7）。这些结果支持将tATP用作饮用水公用事业的有价值的补充水华监测工具，以在监测和治疗蓝藻水华期间实施。
