Abstract：
The manufacturing of indigo naturalis requires prolonged leaf soaking and lime stirring; the resulting indigo purity is less than 3.00% and the yield of indigo (measured in stems and leaves weight) is less than 0.50%, making it unsuitable for use in industrial procedures like printing and dyeing. An enzymatic method of creating indigo without the requirement for lime was investigated in order to generate high purity indigo. Single factor tests were performed to optimize the enzymatic preparation conditions. The findings showed that 60 °C, pH 5.5, 200 mL of leaves extract containing 0.45 mg/mL indican, and a 4:1 ratio of the acidic cellulose (activity: 9000 U/mL, liquid) to indican were the ideal parameters for enzymatic preparation. The yield of indigo was 40.32%, and the contents of indigo and indirubin were 37.37% and 2.30%, respectively. MALDI-TOF-MS in positive ion mode and UPLC-Q-TOF-MS in both positive and negative ion modes were used to analyze indigo extracts from Baphicacanthus cusia(Nees) Bremek by enzymatic preparation. It has been discovered that 13 alkaloids, 5 organic acids, 3 terpenoids, 3 steroids, 2 flavones, and 7 other compounds are present in indigo extracts. The presence of the indigo, indirubin, isorhamnetin, tryptanthrin, indigodole B, and indigodole C determined by UPLC-Q-TOF-MS was verified by MALDI-TOF-MS analysis. The enzymatic preparation of indigo extracts kept the same chemical makeup as conventional indigo naturalis. Thermal analysis and SEM morphology were used to confirm that there was no lime in the indigo extract. During the enzymatic process, Baphicacanthus cusia (Nees) Bremek was employed more effectively, increasing the yield and purity of indigo.
摘要：
天然靛蓝的制造需要长时间的叶片浸泡和石灰搅拌；所得的靛蓝纯度小于3.00％，靛蓝的产率（以茎和叶的重量测量）小于0.50％，使其不适合用于工业程序，如印染。为了产生高纯度的靛蓝，研究了不需要石灰的酶促方法。进行单因素试验以优化酶法制备条件。研究结果表明，60℃，pH5.5，200毫升叶提取物含0.45毫克/毫升印度，和4:1比例的酸性纤维素(活性:9000U/mL，液体）到in是酶法制备的理想参数。靛蓝的收率为40.32%,靛蓝和靛玉红的含量分别为37.37%和2.30%，分别。正离子模式的MALDI-TOF-MS和正离子和负离子模式的UPLC-Q-TOF-MS用于通过酶法制备分析BaphicacanthusCusia（Nees）Bremek的靛蓝提取物。已经发现13种生物碱，5有机酸，3萜类化合物，3类固醇,2个黄酮，和7种其他化合物存在于靛蓝提取物中。靛蓝的存在，靛玉红,异鼠李素,色胺酮,indidoleB,UPLC-Q-TOF-MS测定的吲哚C经MALDI-TOF-MS分析验证。靛蓝提取物的酶法制备保持与常规靛蓝相同的化学组成。热分析和SEM形态用于确认靛蓝提取物中没有石灰。在酶促过程中，Baphicacanthuscusia（Nees）Bremek被更有效地使用，提高靛蓝的产量和纯度。
