Abstract：
Ganoderma lucidum, widely used in traditional medicine, has several biological properties. Polysaccharides, mainly glucans, are known as one of its main bioactive compounds. Consequently, the achievement and chemical investigation of such molecules are of pharmaceutical interest. Herein, we obtained water-insoluble and water-soluble polysaccharides from G. lucidum by alkaline extraction. Fractionation process yielded three fractions (GLC-1, GLC-2, and GLC-3). All samples showed to be composed mainly of glucans. GLC-1 is a linear (1 → 3)-linked β-glucan; GLC-2 is a mixture of three different linear polysaccharides: (1 → 3)-β-glucan, (1 → 3)-α-glucan, and (1 → 4)-α-mannan; while GLC-3 is a branched β-glucan with a (1 → 4)-linked main chain, which is branched at O-3 or O-6 by (1 → 3)- or (1 → 6)-linked side chains. This research reports the variability of glucans in Ganoderma lucidum fruiting bodies and applicable methodologies to obtain such molecules. These polysaccharides can be further applied in biological studies aiming to investigate how their chemical differences may affect their biological properties.
摘要：
灵芝,广泛用于传统医学，具有多种生物学特性。多糖，主要是葡聚糖,被称为其主要的生物活性化合物之一。因此,这些分子的成就和化学研究具有药学意义。在这里，我们通过碱提取从灵芝中获得水不溶性和水溶性多糖。分级分离过程产生三个级分(GLC-1、GLC-2和GLC-3)。所有样品显示主要由葡聚糖组成。GLC-1是线性（1→3）连接的β-葡聚糖；GLC-2是三种不同线性多糖的混合物：（1→3）-β-葡聚糖，(1→3)-α-葡聚糖,和（1→4）-α-甘露聚糖；而GLC-3是具有（1→4）连接的主链的支链β-葡聚糖，其通过(1→3)-或(1→6)-连接的侧链在O-3或O-6处支化。这项研究报道了灵芝子实体中葡聚糖的变异性以及获得此类分子的适用方法。这些多糖可以进一步应用于生物学研究，旨在研究它们的化学差异如何影响它们的生物学特性。
