Abstract：
The efficacy of nanoparticles (NPs) in healthcare applications hinges on their biocidal activity and biocompatibility. This research is dedicated to green-synthesized NPs with potent biocidal properties, aiming for high inhibition rates in bacterial infections and offering a multifunctional application, including potential use in anticancer therapy, in comparison to traditional antibiotics. The present study focuses on synthesis of zinc oxide (ZnO) nanoparticles (NPs), including iron-doped ZnO (GZF) and cobalt-doped ZnO (GZC), using the green co-precipitation method involving Psidium guajava (P. guajava) leaf extract. The physicochemical properties of the synthesized NPs were analyzed using various characterization techniques. The antibacterial and anticancer activity depends on the generation of reactive oxygen species (ROS), particle size, surface area, oxygen vacancy, Zn2+ release, and diffusion ability. The antibacterial activity of the synthesized NPs was tested against various Gram-positive (Streptococcus pneumoniae (S. pneumoniae), Bacillus subtilis (B. subtilis) and Gram-negative (Klebsiella pneumoniae (K. pneumoniae), and Pseudomonas aeruginosa (P. aeruginosa) bacterial strains. The zone of inhibition showed higher activity of GZC (18-20 mm) compared to GZF (16-19 mm) and GZO (11-15 mm) NPs. Moreover, anticancer studies against blood cancer cell line (MOLT-4) showed half-maximal inhibitory concentration of 11.3 μg/mL for GZC compared to GZF and GZO NPs with 12.1 μg/mL and 12.5 μg/mL, respectively. Cytotoxicity assessments carried out on the fibroblast L929 cell line indicated that GZO, GZF, and GZC NPs demonstrated cell viabilities of 85.43%, 86.66%, and 88.14%, respectively. Thus, green-synthesized GZC NPs hold promise as multifunctional agents in the biomedical sector.
摘要：
纳米颗粒(NPs)在医疗保健应用中的功效取决于它们的杀生物活性和生物相容性。这项研究致力于具有有效杀生物特性的绿色合成NP，针对细菌感染的高抑制率，并提供多功能应用，包括在抗癌治疗中的潜在用途，与传统抗生素相比。本研究的重点是氧化锌（ZnO）纳米颗粒（NPs）的合成，包括铁掺杂ZnO（GZF）和钴掺杂ZnO（GZC），使用涉及Psidiumguajava的绿色共沉淀方法(P.guajava)叶提取物。使用各种表征技术分析合成的NP的物理化学性质。抗菌和抗癌活性取决于活性氧（ROS）的产生，颗粒大小,表面积,氧空位,Zn2+释放，和扩散能力。针对各种革兰氏阳性（肺炎链球菌（S.肺炎），枯草芽孢杆菌（B.枯草杆菌）和革兰氏阴性（肺炎克雷伯菌（K。肺炎），和铜绿假单胞菌(P.铜绿假单胞菌）细菌菌株。与GZF(16-19mm)和GZO(11-15mm)NP相比,抑制区显示出更高的GZC(18-20mm)活性。此外，针对血液癌细胞系（MOLT-4）的抗癌研究显示，GZC的半最大抑制浓度为11.3μg/mL，而GZF和GZONP为12.1μg/mL和12.5μg/mL，分别。对成纤维细胞L929细胞系进行的细胞毒性评估表明，GZO，GZF,GZCNPs的细胞活力为85.43%，86.66%,和88.14%，分别。因此，绿色合成的GZCNPs有望成为生物医学领域的多功能药物。
