Abstract：
OBJECTIVE: The aim of this study was to determine clinically and genetically the allergic effects of titanium and poly-ether-ether-ketone (PEEK) implants following loading in rabbit tibias.
METHODS: This study included 18 white New Zealand male rabbits (n = 18) divided evenly into three groups: control, titanium (Ti), and PEEK (P). Clinically, the allergenic effect of titanium and PEEK was investigated by detecting the effect on lymph nodes. Furthermore, RT-PCR and ELISA were used to detect the expression of certain genes IL-6, TNF-α, OPG, RANKL, and RUNX-2 through both types of implants.
RESULTS: Our findings demonstrated that titanium implants induced enlarged lymph nodes, which PEEK did not. Overall, RT-PCR and ELISA techniques revealed that Ti implants had higher expression of the inflammatory genes IL-6 and TNF-α. Ti had the highest expression in OPG findings, while PEEK had the lowest. RANKL expression was highest in the control group and lowest in the PEEK group. RUNX-2 is the highest for the control group and the lowest for the titanium group.
CONCLUSIONS: Although titanium implants elicited greater allergy responses than PEEK implants, titanium has the highest expression of bone formation genes and the lowest expression of bone resorption genes, making it preferable to PEEK.
METHODS: This study included 18 white New Zealand male rabbits (n = 18) divided evenly into three groups: control, titanium (Ti), and PEEK (P). Clinically, the allergenic effect of titanium and PEEK was investigated by detecting the effect on lymph nodes. Furthermore, RT-PCR and ELISA were used to detect the expression of certain genes IL-6, TNF-α, OPG, RANKL, and RUNX-2 through both types of implants.
RESULTS: Our findings demonstrated that titanium implants induced enlarged lymph nodes, which PEEK did not. Overall, RT-PCR and ELISA techniques revealed that Ti implants had higher expression of the inflammatory genes IL-6 and TNF-α. Ti had the highest expression in OPG findings, while PEEK had the lowest. RANKL expression was highest in the control group and lowest in the PEEK group. RUNX-2 is the highest for the control group and the lowest for the titanium group.
CONCLUSIONS: Although titanium implants elicited greater allergy responses than PEEK implants, titanium has the highest expression of bone formation genes and the lowest expression of bone resorption genes, making it preferable to PEEK.
摘要：
目的：这项研究的目的是在临床和遗传上确定钛和聚醚醚酮（PEEK）植入物在兔胫骨中加载后的过敏作用。
方法：这项研究包括18只新西兰白兔（n=18），平均分为三组：对照组，钛(Ti),和PEEK（P）。临床上,通过检测对淋巴结的影响来研究钛和PEEK的致敏作用.此外，RT-PCR和ELISA检测IL-6、TNF-α、OPG,RANKL,和RUNX-2通过两种类型的植入物。
结果：我们的研究结果表明，钛植入物引起淋巴结肿大，PEEK没有。总的来说,RT-PCR和ELISA技术显示，Ti植入物对炎症基因IL-6和TNF-α的表达更高。Ti在OPG发现中表达最高，而PEEK最低。RANKL表达在对照组中最高,在PEEK组中最低。RUNX-2在对照组中最高，在钛组中最低。
结论：尽管钛植入物比PEEK植入物引起更大的过敏反应，钛的骨形成基因表达量最高，骨吸收基因表达量最低，比PEEK更可取。
方法：这项研究包括18只新西兰白兔（n=18），平均分为三组：对照组，钛(Ti),和PEEK（P）。临床上,通过检测对淋巴结的影响来研究钛和PEEK的致敏作用.此外，RT-PCR和ELISA检测IL-6、TNF-α、OPG,RANKL,和RUNX-2通过两种类型的植入物。
结果：我们的研究结果表明，钛植入物引起淋巴结肿大，PEEK没有。总的来说,RT-PCR和ELISA技术显示，Ti植入物对炎症基因IL-6和TNF-α的表达更高。Ti在OPG发现中表达最高，而PEEK最低。RANKL表达在对照组中最高,在PEEK组中最低。RUNX-2在对照组中最高，在钛组中最低。
结论：尽管钛植入物比PEEK植入物引起更大的过敏反应，钛的骨形成基因表达量最高，骨吸收基因表达量最低，比PEEK更可取。
