PDF(Pubmed)
Abstract：
The Asian tiger mosquito, Aedes albopictus, is a global invasive species, notorious for its role in transmitting dangerous human arboviruses such as dengue and Chikungunya. Although hematophagous behavior is repulsive, it is an effective strategy for mosquitoes like Aedes albopictus to transmit viruses, posing a significant risk to human health. However, the fragmented nature of the Ae. albopictus genome assembly has been a significant challenge, hindering in-depth biological and genetic studies of this mosquito. In this research, we have harnessed a variety of technologies and implemented a novel strategy to create a significantly improved genome assembly for Ae. albopictus, designated as AealbF3. This assembly boasts a completeness rate of up to 98.1%, and the duplication rate has been minimized to 1.2%. Furthermore, the fragmented contigs or scaffolds of AealbF3 have been organized into three distinct chromosomes, an arrangement corroborated through syntenic plot analysis, which compared the genetic structure of Ae. albopictus with that of Ae. aegypti. Additionally, the study has revealed a phylogenetic relationship suggesting that the PGANT3 gene is implicated in the hematophagous behavior of Ae. albopictus. This involvement was preliminarily substantiated through RNA interference (RNAi) techniques and behavioral experiment. In summary, the AealbF3 genome assembly will facilitate new biological insights and intervention strategies for combating this formidable vector of disease. The innovative assembly process employed in this study could also serve as a valuable template for the assembly of genomes in other insects characterized by high levels of heterozygosity.
摘要：
亚洲虎蚊子，白纹伊蚊,是一种全球入侵物种，因其在传播登革热和基孔肯雅等危险的人类虫媒病毒方面的作用而臭名昭著。尽管吸血行为是排斥的，对于像白纹伊蚊这样的蚊子来说，这是一种传播病毒的有效策略，对人类健康构成重大风险。然而,Ae的支离破碎的性质。白纹伊蚊基因组组装一直是一个重大挑战，阻碍了对这种蚊子进行深入的生物学和遗传学研究。在这项研究中,我们已经利用了多种技术，并实施了一种新策略，为Ae创建了一种显着改善的基因组组装。白纹,指定为AealbF3。该组件的完成率高达98.1％，重复率已降到1.2%。此外，AealbF3的片段重叠群或支架已被组织成三个不同的染色体，通过同势情节分析证实的安排，比较了Ae的遗传结构。白斑与Ae的白斑。埃及伊蚊.此外,该研究揭示了一种系统发育关系，表明PGANT3基因与Ae的嗜血行为有关。白纹。通过RNA干扰（RNAi）技术和行为实验初步证实了这种参与。总之,AealbF3基因组组装将促进新的生物学见解和干预策略，以对抗这种强大的疾病载体。本研究中采用的创新组装过程也可以作为其他以高水平杂合性为特征的昆虫的基因组组装的有价值的模板。
