Abstract：
Xuebijing injection (XBJ) is widely used to treat nephrotic syndrome (NS) in clinic, but its bioactive components and therapeutic mechanism are still unclear. In this study, the bioactive components of XBJ were determined by ultra-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF/MS). The therapeutic effect of XBJ on NS was evaluated in BALB/c mice induced by adriamycin (ADR, 10 mg/kg) via a single tail vein. The protective effect of XBJ and its bioactive components on podocytes was demonstrated using mouse podocytes (MPC-5) induced by lipopolysaccharide (LPS, 4 μg/mL). The results show that 33 components of XBJ were identified. Furthermore, 12 bioactive components were detected in blood, including protocatechuic acid, salvianolic acid C, benzoyloxypaeoniflorin, danshensu, salvianolic acid A, salvianolic acid B, catechin, caffeic acid, galloylpaeoniflorin, oxypaeoniflorin, hydroxysafflor yellow A, rosmarinic acid. The relative content (%) of the bioactive components were 59.32, 16.01, 9.97, 9.73, 8.72, 8.31, 7.92, 6.54, 1.54, 1.30, 0.68 and 0.59 in this order. After XBJ treatment, the renal function, hyperlipidemia and renal pathological damage were improved in NS model mice. Moreover, the levels of nephrin and desmin which are functional proteins in podocytes were reversed, and the levels of pro-inflammatory factors were reduced by XBJ. Interestingly, protocatechuic acid and salvianolic acid C also showed good protective effects on podocyte function and reduced the level of inflammation in LPS-induced MPC-5. The study is the first time to elucidate the bioactive components of XBJ and its potential therapeutic mechanism for treating NS by protecting podocyte function.
摘要：
血必净注射液(XBJ)在临床上广泛用于治疗肾病综合征(NS),但其生物活性成分和治疗机制尚不清楚。在这项研究中,采用超高效液相色谱-四极杆-飞行时间串联质谱(UPLC-Q-TOF/MS)测定XBJ的生物活性成分。在阿霉素诱导的BALB/c小鼠中评估XBJ对NS的治疗作用（ADR，10mg/kg)经由单尾静脉。使用脂多糖（LPS，4μg/mL)。结果表明,鉴定出XBJ的33个组分。此外，在血液中检测到12种生物活性成分，包括原儿茶酸,丹酚酸C,苯甲酰氧基烟草素，丹参素,丹酚酸A,丹酚酸B,儿茶素,咖啡酸,胆酰芍药苷,氧合烟苷,羟基红花黄色素A，迷迭香酸.生物活性成分的相对含量(%)依次为59.32、16.01、9.97、9.73、8.72、8.31、7.92、6.54、1.54、1.30、0.68和0.59。XBJ治疗后,肾功能，改善NS模型小鼠的高脂血症和肾脏病理损害。此外，足细胞中功能蛋白nephrin和desmin的水平被逆转，XBJ降低促炎因子水平。有趣的是,原儿茶酸和丹酚酸C对LPS诱导的MPC-5中的足细胞功能也具有良好的保护作用，并降低了炎症水平。该研究首次阐明XBJ的生物活性成分及其通过保护足细胞功能治疗NS的潜在治疗机制。
