PDF(Pubmed)
Abstract：
Here, we present a protocol for 3D printing heart tissues using thiol-norbornene photoclick collagen (NorCol). We describe steps for synthesizing NorCol, preparing bioink and the support bath, and cell-laden printing. We then detail procedures for the loading of C2C12 cells into NorCol, ensuring structural integrity and cell viability after printing. This protocol is adaptable to various cell lines and allows for the printing of diverse complex structures, which can be used in drug screening and disease modeling.
摘要：
这里,我们提出了使用硫醇-降冰片烯photocks胶原蛋白（NorCol）进行3D打印心脏组织的方案。我们描述了合成NorCol的步骤，准备生物墨水和支持浴，和充满细胞的打印。然后，我们详细介绍了将C2C12细胞加载到NorCol中的程序，确保打印后的结构完整性和细胞活力。该协议适用于各种细胞系，并允许打印各种复杂结构，可用于药物筛选和疾病建模。
