Abstract：
The ability to recognize a host plant is crucial for insects to meet their nutritional needs and locate suitable sites for laying eggs. Bactrocera dorsalis is a highly destructive pest in fruit crops. Benzothiazole has been found to induce oviposition behavior in the gravid B. dorsalis. However, the ecological roles and the olfactory receptor responsible for benzothiazole are not yet fully understood. In this study, we found that adults were attracted to benzothiazole, which was an effective oviposition stimulant. In vitro experiments showed that BdorOR49b was narrowly tuned to benzothiazole. The electroantennogram results showed that knocking out BdorOR49b significantly reduced the antennal electrophysiological response to benzothiazole. Compared with wild-type flies, the attractiveness of benzothiazole to BdorOR49b knockout adult was significantly attenuated, and mutant females exhibited a severe decrease in oviposition behavior. Altogether, our work provides valuable insights into chemical communications and potential strategies for the control of this pest.
摘要：
识别寄主植物的能力对于昆虫满足其营养需求并找到合适的产卵地点至关重要。背杆菌是水果作物中的一种极具破坏性的害虫。已发现苯并噻唑在妊娠双歧杆菌中诱导产卵行为。然而,生态作用和负责苯并噻唑的嗅觉受体尚未完全了解。在这项研究中,我们发现成年人被苯并噻唑吸引，是一种有效的产卵兴奋剂.体外实验表明，BdorOR49b被狭义地调整为苯并噻唑。触角图结果表明，敲除BdorOR49b可显着降低对苯并噻唑的触角电生理反应。与野生型苍蝇相比，苯并噻唑对BdorOR49b基因敲除成虫的吸引力显著减弱,和突变的雌性表现出严重的产卵行为下降。总之,我们的工作为化学通讯和控制这种害虫的潜在策略提供了有价值的见解。
