PDF(Pubmed)
Abstract：
Brainbow is a genetic cell-labeling technique that allows random colorization of multiple cells and real-time visualization of cell fate within a tissue, providing valuable insights into understanding complex biological processes. However, fluorescent proteins (FPs) in Brainbow have distinct excitation spectra with peak difference greater than 35 nm, which requires sequential imaging under multiple excitations and thus leads to long acquisition times. In addition, they are not easily used together with other fluorophores due to severe spectral bleed-through. Here, we report the development of a single-wavelength excitable Brainbow, UFObow, incorporating three newly developed blue-excitable FPs. We have demonstrated that UFObow enables not only tracking the growth dynamics of tumor cells in vivo but also mapping spatial distribution of immune cells within a sub-cubic centimeter tissue, revealing cell heterogeneity. This provides a powerful means to explore complex biology in a simultaneous imaging manner at a single-cell resolution in organs or in vivo.
摘要：
Brainbow是一种遗传细胞标记技术，可以对多个细胞进行随机着色，并实时显示组织内的细胞命运，为理解复杂的生物过程提供有价值的见解。然而,Brainbow中的荧光蛋白（FPs）具有不同的激发光谱，峰差大于35nm，这需要在多个激励下进行顺序成像，因此导致较长的采集时间。此外,由于严重的光谱出血，它们不容易与其他荧光团一起使用。这里,我们报告了单波长可激发脑弓的发展，UFObow,合并了三个新开发的蓝色兴奋FP。我们已经证明，UFObow不仅可以跟踪体内肿瘤细胞的生长动态，还可以绘制亚立方厘米组织内免疫细胞的空间分布，揭示细胞异质性。这提供了在器官或体内以单细胞分辨率同时成像方式探索复杂生物学的有力手段。
