PDF(Pubmed)
Abstract：
The purpose of this study is to investigate if grape consumption, in the form of grape powder (GP), could protect against ultraviolet (UV)-induced cataract. Mice were fed with the regular diet, sugar placebo diet, or a grape diet (regular diet supplemented with 5%, 10%, and 15% GP) for 3 months. The mice were then exposed to UV radiation to induce cataract. The results showed that the GP diet dose-dependently inhibited UV-induced cataract and preserved glutathione pools. Interestingly, UV-induced Nrf2 activation was abolished in the groups on the GP diet, suggesting GP consumption may improve redox homeostasis in the lens, making Nrf2 activation unnecessary. For molecular target prediction, a total of 471 proteins regulated by GP were identified using Agilent Literature Search (ALS) software. Among these targets, the X-linked inhibitor of apoptosis (XIAP) was correlated with all of the main active ingredients of GP, including resveratrol, catechin, quercetin, and anthocyanins. Our data confirmed that GP prevented UV-induced suppression of XIAP, indicating that XIAP might be one of the critical molecular targets of GP. In conclusion, this study demonstrated that GP protected the lens from UV-induced cataract development in mice. The protective effects of GP may be attributed to its ability to improve redox homeostasis and activate the XIAP-mediated antiapoptotic pathway.
摘要：
这项研究的目的是调查葡萄消费，以葡萄粉（GP）的形式，可以防止紫外线（UV）引起的白内障。用常规饮食喂养小鼠，糖安慰剂饮食，或葡萄饮食（常规饮食补充5％，10%,和15%GP)持续3个月。然后将小鼠暴露于UV辐射以诱导白内障。结果表明，GP饮食剂量依赖性地抑制紫外线诱导的白内障并保留谷胱甘肽库。有趣的是,UV诱导的Nrf2激活在GP饮食的组中被废除，提示服用GP可以改善晶状体的氧化还原稳态，使Nrf2激活不必要。对于分子靶标预测，使用Agilent文献检索(ALS)软件鉴定了由GP调控的471种蛋白质.在这些目标中,X连锁凋亡抑制因子(XIAP)与GP的所有主要活性成分相关,包括白藜芦醇,儿茶素,槲皮素,和花青素.我们的数据证实，GP阻止了紫外线诱导的XIAP抑制，表明XIAP可能是GP的关键分子靶标之一。总之,这项研究表明，GP保护晶状体免受紫外线诱导的小鼠白内障发展。GP的保护作用可能归因于其改善氧化还原稳态和激活XIAP介导的抗凋亡途径的能力。
