PDF(Pubmed)
Abstract：
BACKGROUND: Polynucleotides (PN) are becoming more prominent in aesthetic medicine. However, the structural characteristics of PN have not been published and PN from different companies may have different structural characteristics. This study aimed to elucidate the structural attributes of DOT™ PN and distinguish differences with polydeoxyribonucleotides (PDRN) using high-resolution scanning electron microscopy (SEM) imaging.
METHODS: DOT™ PN was examined using a Quanta 3-D field emission gun (FEG) Scanning Electron Microscope (SEM). Sample preparation involved cryogenic cooling, cleavage, etching, and metal coating to facilitate high-resolution imaging. Cryo-FIB/SEM techniques were employed for in-depth structural analysis.
RESULTS: PDRN exhibited an amorphous structure without distinct features. In contrast, DOT™ PN displayed well-defined polyhedral shapes with smooth, uniformly thick walls. These cells were empty, with diameters ranging from 3 to 8 micrometers, forming a seamless tessellation pattern.
CONCLUSIONS: DOT™ PN\'s distinct geometric tessellation design conforms to the principles of biotensegrity, providing both structural reinforcement and integrity. The presence of delicate partitions and vacant compartments hints at possible uses in the field of pharmaceutical delivery systems. Within the realms of beauty enhancement and regenerative medicine, DOT™ PN\'s capacity to bolster cell growth and tissue mending could potentially transform approaches to rejuvenation treatments. Its adaptability becomes apparent when considering its contributions to drug administration and surgical procedures.
CONCLUSIONS: This study unveils the intricate structural scaffold features of DOT™ PN for the first time, setting it apart from PDRN and inspiring innovation in biomedicine and materials science. DOT™ PN\'s unique attributes open doors to potential applications across healthcare and beyond.
METHODS: DOT™ PN was examined using a Quanta 3-D field emission gun (FEG) Scanning Electron Microscope (SEM). Sample preparation involved cryogenic cooling, cleavage, etching, and metal coating to facilitate high-resolution imaging. Cryo-FIB/SEM techniques were employed for in-depth structural analysis.
RESULTS: PDRN exhibited an amorphous structure without distinct features. In contrast, DOT™ PN displayed well-defined polyhedral shapes with smooth, uniformly thick walls. These cells were empty, with diameters ranging from 3 to 8 micrometers, forming a seamless tessellation pattern.
CONCLUSIONS: DOT™ PN\'s distinct geometric tessellation design conforms to the principles of biotensegrity, providing both structural reinforcement and integrity. The presence of delicate partitions and vacant compartments hints at possible uses in the field of pharmaceutical delivery systems. Within the realms of beauty enhancement and regenerative medicine, DOT™ PN\'s capacity to bolster cell growth and tissue mending could potentially transform approaches to rejuvenation treatments. Its adaptability becomes apparent when considering its contributions to drug administration and surgical procedures.
CONCLUSIONS: This study unveils the intricate structural scaffold features of DOT™ PN for the first time, setting it apart from PDRN and inspiring innovation in biomedicine and materials science. DOT™ PN\'s unique attributes open doors to potential applications across healthcare and beyond.
摘要：
背景：多核苷酸（PN）在美容医学中变得越来越重要。然而,PN的结构特征尚未公布，不同公司的PN可能具有不同的结构特征。本研究旨在阐明DOT™PN的结构属性，并使用高分辨率扫描电子显微镜（SEM）成像区分多脱氧核糖核苷酸（PDRN）的差异。
方法:使用Quanta3-D场发射枪(FEG)扫描电子显微镜(SEM)检查DOT™PN。样品制备包括低温冷却，乳沟,蚀刻,和金属涂层，以促进高分辨率成像。采用Cryo-FIB/SEM技术进行深入的结构分析。
结果：PDRN呈现无明显特征的无定形结构。相比之下,DOT™PN显示轮廓分明的多面体形状，均匀厚壁。这些细胞是空的，直径范围从3到8微米，形成无缝的镶嵌图案。
结论：DOT™PN独特的几何镶嵌设计符合生物整体性原则，提供结构加固和完整性。精巧的分区和空室的存在暗示了在药物递送系统领域中的可能用途。在美容和再生医学领域，DOT™PN支持细胞生长和组织修补的能力可能会改变复兴治疗的方法。当考虑其对药物管理和外科手术的贡献时,其适应性变得明显。
结论：这项研究首次揭示了DOT™PN复杂的结构支架特征，将其与PDRN区分开来，并激发生物医学和材料科学的创新。DOT™PN的独特属性为医疗保健和其他领域的潜在应用打开了大门。
方法:使用Quanta3-D场发射枪(FEG)扫描电子显微镜(SEM)检查DOT™PN。样品制备包括低温冷却，乳沟,蚀刻,和金属涂层，以促进高分辨率成像。采用Cryo-FIB/SEM技术进行深入的结构分析。
结果：PDRN呈现无明显特征的无定形结构。相比之下,DOT™PN显示轮廓分明的多面体形状，均匀厚壁。这些细胞是空的，直径范围从3到8微米，形成无缝的镶嵌图案。
结论：DOT™PN独特的几何镶嵌设计符合生物整体性原则，提供结构加固和完整性。精巧的分区和空室的存在暗示了在药物递送系统领域中的可能用途。在美容和再生医学领域，DOT™PN支持细胞生长和组织修补的能力可能会改变复兴治疗的方法。当考虑其对药物管理和外科手术的贡献时,其适应性变得明显。
结论：这项研究首次揭示了DOT™PN复杂的结构支架特征，将其与PDRN区分开来，并激发生物医学和材料科学的创新。DOT™PN的独特属性为医疗保健和其他领域的潜在应用打开了大门。
