METHODS: A prospective, single-institution, randomized control trial placed consented postmastectomy fat grafting patients into 1 of 3 treatment arms (active filtration, low-pressure decantation, and standard decantation) in a 1:1:1 ratio. A preoperative 3-dimensional scan of the upper torso was taken as baseline. At the 3-month postoperative visit, another 3D scan was taken. Audodesk Meshmixer was used to evaluate the volume change.
RESULTS: The volume of fat injected during the initial procedure did not differ significantly between the treatment arms (P > 0.05). Both active filtration and low-pressure decantation resulted in higher percentage volume retention than traditional decantation (P < 0.05). Active filtration and low-pressure decantation exhibited comparable degrees of fat maintenance at 3 months (P > 0.05).
CONCLUSIONS: Compared with using traditional decantation as the lipoaspirate purification technique, active filtration and low-pressure decantation may have led to higher levels of cell viability by way of reduced cellular debris and other inflammatory components that may contribute to tissue resorption and necrosis. Further immunohistochemistry studies are needed to examine whether active filtration and low-pressure decantation lead to lipoaspirates with more concentrated viable adipocytes, progenitor cells, and factors for angiogenesis.
方法:前瞻性,单一机构,随机对照试验将同意的乳房切除术后脂肪移植患者放入3个治疗组中的1个(主动过滤,低压倾析,和标准倾析),比例为1:1:1。术前对上躯干进行三维扫描作为基线。术后3个月访视时,进行了另一次3D扫描。使用AudodeskMeshmixer评估体积变化。
结果:在初始过程中注射的脂肪体积在治疗组之间没有显着差异(P>0.05)。主动过滤和低压倾析均导致比传统倾析更高的体积保留百分比(P<0.05)。主动过滤和低压倾析在3个月时表现出相当程度的脂肪维持(P>0.05)。
结论:与使用传统倾析作为脂肪抽吸物纯化技术相比,主动过滤和低压倾析可能通过减少细胞碎片和其他可能导致组织吸收和坏死的炎症成分而导致更高水平的细胞活力。需要进一步的免疫组织化学研究来检查主动过滤和低压倾析是否导致脂肪抽吸物具有更浓缩的活脂肪细胞。祖细胞,和血管生成的因素。