Abstract：
BACKGROUND: Autologous fat grafting is a method of improving aesthetic outcomes after both breast reconstruction and aesthetic surgery through volume enhancement and tissue contouring. Long-lasting effects are linked to greater patient satisfaction and more optimal augmentation results. Harvesting, processing, and injection techniques may all affect the longevity of deformity filling. Our objective is to evaluate the effect of lipoaspirate processing modality on longitudinal volume retention after surgery.
METHODS: A prospective, single-institution, randomized control trial placed consented postmastectomy fat grafting patients into 1 of 3 treatment arms (active filtration, low-pressure decantation, and standard decantation) in a 1:1:1 ratio. A preoperative 3-dimensional scan of the upper torso was taken as baseline. At the 3-month postoperative visit, another 3D scan was taken. Audodesk Meshmixer was used to evaluate the volume change.
RESULTS: The volume of fat injected during the initial procedure did not differ significantly between the treatment arms (P > 0.05). Both active filtration and low-pressure decantation resulted in higher percentage volume retention than traditional decantation (P < 0.05). Active filtration and low-pressure decantation exhibited comparable degrees of fat maintenance at 3 months (P > 0.05).
CONCLUSIONS: Compared with using traditional decantation as the lipoaspirate purification technique, active filtration and low-pressure decantation may have led to higher levels of cell viability by way of reduced cellular debris and other inflammatory components that may contribute to tissue resorption and necrosis. Further immunohistochemistry studies are needed to examine whether active filtration and low-pressure decantation lead to lipoaspirates with more concentrated viable adipocytes, progenitor cells, and factors for angiogenesis.
METHODS: A prospective, single-institution, randomized control trial placed consented postmastectomy fat grafting patients into 1 of 3 treatment arms (active filtration, low-pressure decantation, and standard decantation) in a 1:1:1 ratio. A preoperative 3-dimensional scan of the upper torso was taken as baseline. At the 3-month postoperative visit, another 3D scan was taken. Audodesk Meshmixer was used to evaluate the volume change.
RESULTS: The volume of fat injected during the initial procedure did not differ significantly between the treatment arms (P > 0.05). Both active filtration and low-pressure decantation resulted in higher percentage volume retention than traditional decantation (P < 0.05). Active filtration and low-pressure decantation exhibited comparable degrees of fat maintenance at 3 months (P > 0.05).
CONCLUSIONS: Compared with using traditional decantation as the lipoaspirate purification technique, active filtration and low-pressure decantation may have led to higher levels of cell viability by way of reduced cellular debris and other inflammatory components that may contribute to tissue resorption and necrosis. Further immunohistochemistry studies are needed to examine whether active filtration and low-pressure decantation lead to lipoaspirates with more concentrated viable adipocytes, progenitor cells, and factors for angiogenesis.
摘要：
背景：自体脂肪移植是一种通过体积增强和组织轮廓改善乳房重建和美学手术后的美学结果的方法。持久的效果与更高的患者满意度和更优化的增强结果有关。收割,processing,和注射技术都可能影响畸形填充的寿命。我们的目标是评估脂肪抽吸处理方式对手术后纵向体积保留的影响。
方法：前瞻性，单一机构，随机对照试验将同意的乳房切除术后脂肪移植患者放入3个治疗组中的1个(主动过滤，低压倾析，和标准倾析），比例为1：1：1。术前对上躯干进行三维扫描作为基线。术后3个月访视时,进行了另一次3D扫描。使用AudodeskMeshmixer评估体积变化。
结果：在初始过程中注射的脂肪体积在治疗组之间没有显着差异（P>0.05）。主动过滤和低压倾析均导致比传统倾析更高的体积保留百分比（P<0.05）。主动过滤和低压倾析在3个月时表现出相当程度的脂肪维持(P>0.05)。
结论：与使用传统倾析作为脂肪抽吸物纯化技术相比，主动过滤和低压倾析可能通过减少细胞碎片和其他可能导致组织吸收和坏死的炎症成分而导致更高水平的细胞活力。需要进一步的免疫组织化学研究来检查主动过滤和低压倾析是否导致脂肪抽吸物具有更浓缩的活脂肪细胞。祖细胞，和血管生成的因素。
方法：前瞻性，单一机构，随机对照试验将同意的乳房切除术后脂肪移植患者放入3个治疗组中的1个(主动过滤，低压倾析，和标准倾析），比例为1：1：1。术前对上躯干进行三维扫描作为基线。术后3个月访视时,进行了另一次3D扫描。使用AudodeskMeshmixer评估体积变化。
结果：在初始过程中注射的脂肪体积在治疗组之间没有显着差异（P>0.05）。主动过滤和低压倾析均导致比传统倾析更高的体积保留百分比（P<0.05）。主动过滤和低压倾析在3个月时表现出相当程度的脂肪维持(P>0.05)。
结论：与使用传统倾析作为脂肪抽吸物纯化技术相比，主动过滤和低压倾析可能通过减少细胞碎片和其他可能导致组织吸收和坏死的炎症成分而导致更高水平的细胞活力。需要进一步的免疫组织化学研究来检查主动过滤和低压倾析是否导致脂肪抽吸物具有更浓缩的活脂肪细胞。祖细胞，和血管生成的因素。
