PDF(Pubmed)
Abstract：
Streptomyces species are best known for their ability to produce abundant secondary metabolites with versatile bioactivities and industrial importance. These metabolites are usually biosynthesized through metabolic pathways encoded by cluster-situated genes. These genes are also known as biosynthetic gene clusters (BGCs) of secondary metabolites. The expression of BGCs is intricately controlled by pyramidal transcriptional regulatory cascades, which include various regulators. Streptomyces antibiotic regulatory proteins (SARPs), a genus-specific family of regulators, are widely distributed and play important roles in regulating the biosynthesis of secondary metabolites in Streptomyces. Over the past decade, the biological functions of SARPs have been extensively investigated. Here, we summarized the recent advances in characterizing the roles of SARPs involved in Streptomyces secondary metabolism from the following three aspects. First, the classification and domain organization of SARPs were summarized according to their size variation. Second, we presented a detailed description of the regulatory mechanisms and modes of action of SARPs involved in secondary metabolism. Finally, the biotechnological application of SARPs was illustrated by improving the production of target secondary metabolites and discovering novel bioactive natural products. This review will help researchers to comprehensively understand the roles of SARPs in secondary metabolite biosynthesis in Streptomyces, which will contribute to building a solid foundation for their future application in synthetic biology.
摘要：
链霉菌物种以其产生具有多种生物活性和工业重要性的丰富次生代谢产物的能力而闻名。这些代谢物通常通过位于簇的基因编码的代谢途径进行生物合成。这些基因也被称为次级代谢物的生物合成基因簇(BGC)。BGC的表达是由锥体转录调控级联复杂的控制，其中包括各种监管机构。链霉菌属抗生素调节蛋白（SARPs），一个属特定的调节子家族，链霉菌分布广泛,在调节次生代谢产物的生物合成中起重要作用。在过去的十年里，SARPs的生物学功能已被广泛研究。这里,我们从以下三个方面总结了SARPs参与链霉菌次生代谢的研究进展。首先,根据大小变化对SARPs的分类和区域组织进行了总结。第二,我们详细介绍了参与次级代谢的SARPs的调节机制和作用模式.最后,通过改善目标次生代谢产物的生产和发现新型生物活性天然产物来说明SARPs的生物技术应用。这篇综述将有助于研究人员全面了解SARPs在链霉菌次生代谢产物生物合成中的作用。这将有助于为它们在合成生物学中的未来应用奠定坚实的基础。
