UNASSIGNED: Blood samples were collected from 275 domestic companion animals (117 dogs and 158 cats) living in Tianjin city, China, and the presence of DNA from Anaplasma, Babesia, Bartonella, and Rickettsia was detected by semi-nested polymerase chain reaction (PCR). The PCR products of the expected size were sequenced, and these newly generated sequences were subjected to BLASTN, nucleotide identity, and phylogenetic analyses.
UNASSIGNED: A total of 24 blood samples tested positive for vector-borne pathogens in companion dogs and cats in Tianjin city, China, with a relatively low positive rate of 8.7%. Specifically, seven human pathogens, including Rickettsia raoultii, Candidatus Rickettsia jingxinensis, Rickettsia sibirica, Rickettsia felis, Babesia venatorum, Bartonella tribocorum, and Bartonella Henselae, were identified. In addition, Anaplasma ovis with zoonotic potential and Candidatus A. cinensis were detected.
UNASSIGNED: Our results indicate substantial genetic diversity in the vector-borne human pathogens circulating in companion dogs and cats. Interventions based on \"One Health\" should be taken to reduce the potential risks of contracting infection from companion dogs and cats in Tianjin, China.
■从居住在天津市的275只家养动物(117只狗和158只猫)中采集了血液样本,中国,以及无性体DNA的存在,巴贝西亚,巴尔通体,通过半巢式聚合酶链反应(PCR)检测立克次体。对预期大小的PCR产物进行测序,这些新产生的序列经过BLASTN,核苷酸身份,和系统发育分析。
■天津市共有24份血液样本在伴侣犬和猫中检测出媒介传播病原体阳性,中国,阳性率相对较低,为8.7%。具体来说,七种人类病原体,包括Rickettsiaraoultii,晶心立克次体,Sibirica立克次体,立克次体费利斯,VenatorumBabesia,三角肌巴尔通体,和汉斯莱巴尔通菌,已确定。此外,检测到具有人畜共患潜力的无性体和念珠菌。
■我们的结果表明,在伴侣犬和猫中传播的媒介传播的人类病原体具有大量的遗传多样性。应采取以"一个健康"为基础的干预措施,以降低天津伴侣犬猫感染的潜在风险,中国。