PDF(Pubmed)
Abstract：
UNASSIGNED: MicroRNAs are small noncoding genes with gene expression regulatory function. Their emergence as potential diagnostic biomarker for many diseases has gained a specific interest among researchers. Observations of changes in miRNA levels correlating with aneuploidy in early embryos raise the prospective of employing miRNA as biomarkers to assess the embryo quality.
UNASSIGNED: To identify and gather the miRNAs with potential link to chromosomal abnormalities in embryos from previous research, we conducted a systematic search using four databases, including Embase, Medline, Web of Science, and Cochrane databases in accordance with PRISMA guidelines.
UNASSIGNED: Out of 200 identified records, only seven met the inclusion criteria. Seven miRNAs: miR-19b, miR-517c, miR-518e, miR-522, miR-92a, and miR-106a exhibited persistent downregulation in aneuploid blastocysts in the included studies. These miRNAs are members of important miRNA clusters, associated with abnormal expression in studies on reproductive failure. Pathway analysis revealed their involvement in regulating gene transcription, as well as cell cycle progression and apoptosis.
UNASSIGNED: The changes detected in the miRNA expression in aneuploid embryos across different studies support the aneuploidy and miRNA relationship and prospect miRNA as a valuable tool for the assessment of embryo quality. Collectively, these observations highlight the role of miRNAs in embryonic development, and their involvement in genetic abnormalities that occur in embryos, such as aneuploidy, indicating their potential implementation to improve the embryo selection and reproductive outcomes.
UNASSIGNED: To identify and gather the miRNAs with potential link to chromosomal abnormalities in embryos from previous research, we conducted a systematic search using four databases, including Embase, Medline, Web of Science, and Cochrane databases in accordance with PRISMA guidelines.
UNASSIGNED: Out of 200 identified records, only seven met the inclusion criteria. Seven miRNAs: miR-19b, miR-517c, miR-518e, miR-522, miR-92a, and miR-106a exhibited persistent downregulation in aneuploid blastocysts in the included studies. These miRNAs are members of important miRNA clusters, associated with abnormal expression in studies on reproductive failure. Pathway analysis revealed their involvement in regulating gene transcription, as well as cell cycle progression and apoptosis.
UNASSIGNED: The changes detected in the miRNA expression in aneuploid embryos across different studies support the aneuploidy and miRNA relationship and prospect miRNA as a valuable tool for the assessment of embryo quality. Collectively, these observations highlight the role of miRNAs in embryonic development, and their involvement in genetic abnormalities that occur in embryos, such as aneuploidy, indicating their potential implementation to improve the embryo selection and reproductive outcomes.
摘要：
■MicroRNAs是具有基因表达调控功能的小的非编码基因。它们作为许多疾病的潜在诊断生物标志物的出现引起了研究人员的特殊兴趣。早期胚胎中与非整倍性相关的miRNA水平变化的观察提高了采用miRNA作为生物标志物来评估胚胎质量的前景。
为了从先前的研究中鉴定和收集与胚胎染色体异常有潜在联系的miRNAs，我们使用四个数据库进行了系统的搜索，包括Embase,Medline,WebofScience,和Cochrane数据库符合PRISMA指南。
■在200条确定的记录中，只有7人符合纳入标准。七个miRNA：miR-19b,miR-517c,miR-518e,miR-522,miR-92a,在纳入的研究中,miR-106a在非整倍体囊胚中表现出持续下调.这些miRNA是重要的miRNA簇的成员,与生殖障碍研究中的异常表达有关。路径分析显示它们参与调节基因转录，以及细胞周期进程和细胞凋亡。
■在不同研究中检测到的非整倍体胚胎中miRNA表达的变化支持非整倍体和miRNA的关系，并展望miRNA作为评估胚胎质量的有价值的工具。总的来说，这些观察强调了miRNAs在胚胎发育中的作用，以及它们参与胚胎中发生的遗传异常，如非整倍性，表明它们的潜在实施，以改善胚胎选择和生殖结果。
为了从先前的研究中鉴定和收集与胚胎染色体异常有潜在联系的miRNAs，我们使用四个数据库进行了系统的搜索，包括Embase,Medline,WebofScience,和Cochrane数据库符合PRISMA指南。
■在200条确定的记录中，只有7人符合纳入标准。七个miRNA：miR-19b,miR-517c,miR-518e,miR-522,miR-92a,在纳入的研究中,miR-106a在非整倍体囊胚中表现出持续下调.这些miRNA是重要的miRNA簇的成员,与生殖障碍研究中的异常表达有关。路径分析显示它们参与调节基因转录，以及细胞周期进程和细胞凋亡。
■在不同研究中检测到的非整倍体胚胎中miRNA表达的变化支持非整倍体和miRNA的关系，并展望miRNA作为评估胚胎质量的有价值的工具。总的来说，这些观察强调了miRNAs在胚胎发育中的作用，以及它们参与胚胎中发生的遗传异常，如非整倍性，表明它们的潜在实施，以改善胚胎选择和生殖结果。
