Abstract：
Bisabolene is a compound commonly found in essential oils of various plants. It has a broad application in sectors such as chemical, pharmaceutical, and health-care products. This study focuses on modifying the glycerol metabolism pathway to obtain a high bisabolene-producing strain of Saccharomyces cerevisiae. To achieve this, the glycerol transporter gene PtFPS2 from Pachysolen tannophilus and the glycerol dehydrogenase gene Opgdh from Ogataea parapolymorpha were overexpressed in engineered yeast YS036, which was equipped with a GAL promoters-enhanced mevalonic acid pathway. Additionally, the glucose-inhibiting transcription factor MIG1 was knocked out to reduce glucose inhibition. The results showed that the GAL promoter transcription levels of the recombinant yeast strains increased, and the co-utilization of sucrose and glycerol was further improved in MIG1-knockout strain. Moreover, the maximum yield of bisabolene in shaking flask fermentation increased to 866.7 mg/L, an 82.2% increase compared to that of the original strain. By modifying the metabolic pathway of carbon sources, the yield of bisabolene was considerably improved. This study offers an effective strategy for enhancing the yield of terpene compounds in engineered yeast.
摘要：
没药烯是一种常见于各种植物精油中的化合物。它在化工等行业有广泛的应用，Pharmaceutical,和保健产品。本研究主要通过对甘油代谢途径的改造，获得一株高产双黑酚的酿酒酵母菌株。为了实现这一点，Pachysolentannophilus的甘油转运蛋白基因PtFPS2和Ogataeapparaporpha的甘油脱氢酶基因Opgdh在工程酵母YS036中过表达，该酵母配备了GAL启动子增强的甲羟戊酸途径。此外,敲除葡萄糖抑制转录因子MIG1以减少葡萄糖抑制.结果表明,重组酵母菌株GAL启动子转录水平升高,在MIG1敲除菌株中，蔗糖和甘油的共利用进一步提高。此外，摇瓶发酵的最大产量增加到866.7mg/L，与原始菌株相比增加了82.2%。通过改变碳源的代谢途径,二黑酚的产量大大提高。本研究为提高工程酵母中萜类化合物的产量提供了有效的策略。
