PDF(Pubmed)
Abstract：
UNASSIGNED: Obesity is a chronic condition associated with low-grade inflammation mainly due to immune cell infiltration of white adipose tissue (WAT). WAT is distributed into two main depots: subcutaneous WAT (sWAT) and visceral WAT (vWAT), each with different biochemical features and metabolic roles. Proinflammatory cytokines including interleukin (IL)-16 are secreted by both adipocytes and infiltrated immune cells to upregulate inflammation. IL-16 has been widely studied in the peripheral proinflammatory immune response; however, little is known about its role in adipocytes in the context of obesity.
UNASSIGNED: We aimed to study the levels of IL-16 in WAT derived from sWAT and vWAT depots of humans with obesity and the role of this cytokine in palmitate-exposed 3T3-L1 adipocytes.
UNASSIGNED: The results demonstrated that IL-16 expression was higher in vWAT compared with sWAT in individuals with obesity. In addition, IL-16 serum levels were higher in patients with obesity compared with normal-weight individuals, increased at 6 months after bariatric surgery, and at 12 months after surgery decreased to levels similar to before the intervention. Our in vitro models showed that IL-16 could modulate markers of adipogenesis (Pref1), lipid metabolism (Plin1, Cd36, and Glut4), fibrosis (Hif1a, Col4a, Col6a, and Vegf), and inflammatory signaling (IL6) during adipogenesis and in mature adipocytes. In addition, lipid accumulation and glycerol release assays suggested lipolysis alteration.
UNASSIGNED: Our results suggest a potential role of IL-16 in adipogenesis, lipid and glucose homeostasis, fibrosis, and inflammation in an obesity context.
UNASSIGNED: We aimed to study the levels of IL-16 in WAT derived from sWAT and vWAT depots of humans with obesity and the role of this cytokine in palmitate-exposed 3T3-L1 adipocytes.
UNASSIGNED: The results demonstrated that IL-16 expression was higher in vWAT compared with sWAT in individuals with obesity. In addition, IL-16 serum levels were higher in patients with obesity compared with normal-weight individuals, increased at 6 months after bariatric surgery, and at 12 months after surgery decreased to levels similar to before the intervention. Our in vitro models showed that IL-16 could modulate markers of adipogenesis (Pref1), lipid metabolism (Plin1, Cd36, and Glut4), fibrosis (Hif1a, Col4a, Col6a, and Vegf), and inflammatory signaling (IL6) during adipogenesis and in mature adipocytes. In addition, lipid accumulation and glycerol release assays suggested lipolysis alteration.
UNASSIGNED: Our results suggest a potential role of IL-16 in adipogenesis, lipid and glucose homeostasis, fibrosis, and inflammation in an obesity context.
摘要：
■肥胖是一种与低度炎症相关的慢性疾病，主要是由于白色脂肪组织（WAT）的免疫细胞浸润。WAT分为两个主要仓库：皮下WAT（sWAT）和内脏WAT（vWAT），每个都有不同的生化特征和代谢作用。包括白介素(IL)-16的促炎细胞因子由脂肪细胞和浸润的免疫细胞分泌以上调炎症。IL-16在外周促炎免疫反应中已被广泛研究；然而，关于它在肥胖背景下的脂肪细胞中的作用知之甚少。
■我们旨在研究来自肥胖人类的sWAT和vWAT储库的WAT中IL-16的水平，以及该细胞因子在棕榈酸暴露的3T3-L1脂肪细胞中的作用。
■结果表明，与肥胖个体相比，vWAT中IL-16的表达更高。此外,与体重正常的人相比，肥胖患者的IL-16血清水平更高，在减肥手术后6个月增加，在手术后12个月下降到与干预前相似的水平。我们的体外模型显示，IL-16可以调节脂肪形成的标志物（Pref1），脂质代谢（Plin1，Cd36和Glut4），纤维化(Hif1a，Col4a,Col6a,和Vegf），和脂肪生成期间和成熟脂肪细胞中的炎症信号(IL6)。此外,脂质积累和甘油释放试验表明脂解改变。
■我们的结果表明，IL-16在脂肪形成中具有潜在的作用，脂质和葡萄糖稳态，纤维化，和肥胖背景下的炎症。
■我们旨在研究来自肥胖人类的sWAT和vWAT储库的WAT中IL-16的水平，以及该细胞因子在棕榈酸暴露的3T3-L1脂肪细胞中的作用。
■结果表明，与肥胖个体相比，vWAT中IL-16的表达更高。此外,与体重正常的人相比，肥胖患者的IL-16血清水平更高，在减肥手术后6个月增加，在手术后12个月下降到与干预前相似的水平。我们的体外模型显示，IL-16可以调节脂肪形成的标志物（Pref1），脂质代谢（Plin1，Cd36和Glut4），纤维化(Hif1a，Col4a,Col6a,和Vegf），和脂肪生成期间和成熟脂肪细胞中的炎症信号(IL6)。此外,脂质积累和甘油释放试验表明脂解改变。
■我们的结果表明，IL-16在脂肪形成中具有潜在的作用，脂质和葡萄糖稳态，纤维化，和肥胖背景下的炎症。
