UNASSIGNED: We aimed to study the levels of IL-16 in WAT derived from sWAT and vWAT depots of humans with obesity and the role of this cytokine in palmitate-exposed 3T3-L1 adipocytes.
UNASSIGNED: The results demonstrated that IL-16 expression was higher in vWAT compared with sWAT in individuals with obesity. In addition, IL-16 serum levels were higher in patients with obesity compared with normal-weight individuals, increased at 6 months after bariatric surgery, and at 12 months after surgery decreased to levels similar to before the intervention. Our in vitro models showed that IL-16 could modulate markers of adipogenesis (Pref1), lipid metabolism (Plin1, Cd36, and Glut4), fibrosis (Hif1a, Col4a, Col6a, and Vegf), and inflammatory signaling (IL6) during adipogenesis and in mature adipocytes. In addition, lipid accumulation and glycerol release assays suggested lipolysis alteration.
UNASSIGNED: Our results suggest a potential role of IL-16 in adipogenesis, lipid and glucose homeostasis, fibrosis, and inflammation in an obesity context.
■我们旨在研究来自肥胖人类的sWAT和vWAT储库的WAT中IL-16的水平,以及该细胞因子在棕榈酸暴露的3T3-L1脂肪细胞中的作用。
■结果表明,与肥胖个体相比,vWAT中IL-16的表达更高。此外,与体重正常的人相比,肥胖患者的IL-16血清水平更高,在减肥手术后6个月增加,在手术后12个月下降到与干预前相似的水平。我们的体外模型显示,IL-16可以调节脂肪形成的标志物(Pref1),脂质代谢(Plin1,Cd36和Glut4),纤维化(Hif1a,Col4a,Col6a,和Vegf),和脂肪生成期间和成熟脂肪细胞中的炎症信号(IL6)。此外,脂质积累和甘油释放试验表明脂解改变。
■我们的结果表明,IL-16在脂肪形成中具有潜在的作用,脂质和葡萄糖稳态,纤维化,和肥胖背景下的炎症。