PDF(Pubmed)
Abstract：
UNASSIGNED: Motilin is a hormone secreted by specialised enteroendocrine cells in the small intestine, and is known to modulate gastrointestinal motility in humans, regulating the migratory motor complex. It is understudied at least in part due to the lack of commercially available immunoassays.
UNASSIGNED: A multiplexed liquid chromatography mass spectrometry (LC-MS/MS) method was optimised to measure motilin, insulin, C-peptide, GIP (1-42) and GIP (3-42). Corresponding active ghrelin concentrations were determined by immunoassay. Ten healthy volunteers with no prior history of gastroenterological or endocrine condition attended after overnight fast and had blood samples taken every 15 minutes for 4 hours whilst continuing to fast, and then further sampling for 2 hours following a liquid mixed meal. Hunger scores were taken at each time point using a visual analogue scale. Normal bowel habit was confirmed by 1 week stool diary.
UNASSIGNED: Motilin levels fluctuated in the fasting state with an average period between peaks of 109.5 mins (SD:30.0), but with no evidence of a relationship with either ghrelin levels or hunger scores. The mixed meal interrupted cyclical motilin fluctuations, increased concentrations of motilin, insulin, C-peptide, GIP(1-42) and GIP(3-42), and suppressed ghrelin levels.
UNASSIGNED: This study highlights the utility of LC-MS/MS for parallel measurement of motilin alongside other peptide hormones, and supports previous reports of the cyclical nature of motilin levels in the fasting state and interruption with feeding. This analytical method has utility for further clinical studies into motilin and gut hormone physiology in human volunteers.
UNASSIGNED: A multiplexed liquid chromatography mass spectrometry (LC-MS/MS) method was optimised to measure motilin, insulin, C-peptide, GIP (1-42) and GIP (3-42). Corresponding active ghrelin concentrations were determined by immunoassay. Ten healthy volunteers with no prior history of gastroenterological or endocrine condition attended after overnight fast and had blood samples taken every 15 minutes for 4 hours whilst continuing to fast, and then further sampling for 2 hours following a liquid mixed meal. Hunger scores were taken at each time point using a visual analogue scale. Normal bowel habit was confirmed by 1 week stool diary.
UNASSIGNED: Motilin levels fluctuated in the fasting state with an average period between peaks of 109.5 mins (SD:30.0), but with no evidence of a relationship with either ghrelin levels or hunger scores. The mixed meal interrupted cyclical motilin fluctuations, increased concentrations of motilin, insulin, C-peptide, GIP(1-42) and GIP(3-42), and suppressed ghrelin levels.
UNASSIGNED: This study highlights the utility of LC-MS/MS for parallel measurement of motilin alongside other peptide hormones, and supports previous reports of the cyclical nature of motilin levels in the fasting state and interruption with feeding. This analytical method has utility for further clinical studies into motilin and gut hormone physiology in human volunteers.
摘要：
■胃动素是由小肠中专门的肠内分泌细胞分泌的激素，并且已知调节人类的胃肠蠕动，调节迁徙运动复合体。至少部分地由于缺乏可商购的免疫测定而对其研究不足。
■优化了多重液相色谱质谱（LC-MS/MS）方法来测量胃动素，胰岛素，C-肽，GIP（1-42）和GIP（3-42）。通过免疫测定确定相应的活性生长素释放肽浓度。10名没有胃肠病或内分泌疾病史的健康志愿者在禁食过夜后就诊，每15分钟采集一次血样，持续禁食4小时，然后在液体混合膳食后进一步取样2小时。在每个时间点使用视觉模拟量表进行饥饿评分。通过1周粪便日记确认正常排便习惯。
■胃动素水平在空腹状态下波动，平均时间在109.5分钟的峰值之间（SD：30.0），但没有证据表明与饥饿水平或饥饿分数有关。混合餐中断了周期性的胃动素波动，胃动素浓度增加，胰岛素，C-肽，GIP（1-42）和GIP（3-42），抑制生长素释放肽水平。
■这项研究强调了LC-MS/MS与其他肽类激素一起平行测量胃动素的实用性，并支持先前关于禁食状态下胃动素水平的周期性和进食中断的报告。该分析方法可用于对人类志愿者的胃动素和肠激素生理学的进一步临床研究。
■优化了多重液相色谱质谱（LC-MS/MS）方法来测量胃动素，胰岛素，C-肽，GIP（1-42）和GIP（3-42）。通过免疫测定确定相应的活性生长素释放肽浓度。10名没有胃肠病或内分泌疾病史的健康志愿者在禁食过夜后就诊，每15分钟采集一次血样，持续禁食4小时，然后在液体混合膳食后进一步取样2小时。在每个时间点使用视觉模拟量表进行饥饿评分。通过1周粪便日记确认正常排便习惯。
■胃动素水平在空腹状态下波动，平均时间在109.5分钟的峰值之间（SD：30.0），但没有证据表明与饥饿水平或饥饿分数有关。混合餐中断了周期性的胃动素波动，胃动素浓度增加，胰岛素，C-肽，GIP（1-42）和GIP（3-42），抑制生长素释放肽水平。
■这项研究强调了LC-MS/MS与其他肽类激素一起平行测量胃动素的实用性，并支持先前关于禁食状态下胃动素水平的周期性和进食中断的报告。该分析方法可用于对人类志愿者的胃动素和肠激素生理学的进一步临床研究。
