PDF(Pubmed)
Abstract：
Foot-and-mouth disease (FMD) is a highly contagious viral infection causing acute and severe vesicular lesions in cattle and pigs, which has prompted global vaccination policies. This study presents a technique for enhancing antigen yield in SAT1 BOT and SAT3 ZIM by treatment with calcium chloride (CaCl2). We tested changes in cell viability in BHK-21 suspension cells treated with varying concentrations of CaCl2. The optimal CaCl2 concentration was determined based on antigen yield. The timing of CaCl2 supplementation relative to FMD virus inoculation was tested. Finally, the optimal medium for antigen production was identified. We observed a concentration-dependent decrease in BHK-21 cell viability at >7.5 mM CaCl2. A CaCl2 concentration of 3 mM yielded the most antigens. CaCl2 supplementation relative to FMD virus infection was optimal 2 h before or with viral inoculation. CD-BHK 21 medium supplemented with CaCl2 was the most productive medium. Specifically, SAT1 BOT and SAT3 ZIM showed improved antigen production in CD-BHK 21 medium with 3 mM CaCl2, while Provero-1 and Cellvento BHK-200 media showed no significant enhancement. Overall, CaCl2 supplementation enhanced FMD antigen productivity. This study provides a useful framework for enhancing antigen production efficiently in the FMD vaccine industry.
摘要：
口蹄疫（FMD）是一种高度传染性的病毒感染，在牛和猪中引起急性和严重的水泡病变，这促使全球疫苗接种政策。这项研究提出了一种通过用氯化钙（CaCl2）处理来提高SAT1BOT和SAT3ZIM中抗原产量的技术。我们测试了用不同浓度的CaCl2处理的BHK-21悬浮细胞中细胞活力的变化。基于抗原产量确定最佳CaCl2浓度。测试了CaCl2补充相对于FMD病毒接种的时机。最后,确定了产生抗原的最佳培养基。我们观察到在>7.5mMCaCl2时BHK-21细胞活力的浓度依赖性降低。3mM的CaCl2浓度产生最多的抗原。相对于FMD病毒感染的CaCl2补充在病毒接种前2小时或与病毒接种一起是最佳的。补充有CaCl2的CD-BHK21培养基是最有生产力的培养基。具体来说,SAT1BOT和SAT3ZIM在含有3mMCaCl2的CD-BHK21培养基中显示出提高的抗原产量，而Provero-1和CellventoBHK-200培养基则没有显着增强。总的来说,补充CaCl2可提高FMD抗原的生产率。这项研究为在口蹄疫疫苗行业中有效增强抗原生产提供了有用的框架。
