PDF(Pubmed)
Abstract：
Wild rodents are considered to be one of the most important TBEV-amplifying reservoir hosts; therefore, they may be suitable for foci detection studies. To investigate the effectiveness of viral RNA detection in wild rodents for suspected TBEV foci confirmation, we trapped small rodents (n = 139) in various locations in Lithuania where TBEV was previously detected in questing ticks. Murine neuroblastoma Neuro-2a cells were inoculated with each rodent sample to maximize the chances of detecting viral RNA in rodent samples. TBEV RNA was detected in 74.8% (CI 95% 66.7-81.1) of the brain and/or internal organ mix suspensions, and the prevalence rate increased significantly following sample cultivation in Neuro-2a cells. Moreover, a strong correlation (r = 0.88; p < 0.05) was found between the average monthly air temperature of rodent trapping and the TBEV RNA prevalence rate in cell culture isolates of rodent suspensions, which were PCR-negative before cultivation in cell culture. This study shows that wild rodents are suitable sentinel animals to confirm TBEV foci. In addition, the study results demonstrate that sample cultivation in cell culture is a highly efficient method for increasing TBEV viral load to detectable quantities.
摘要：
野生啮齿动物被认为是最重要的TBEV扩增水库宿主之一；因此，它们可能适用于病灶检测研究。为了研究病毒RNA检测在野生啮齿动物中用于可疑TBEV病灶确认的有效性，我们在立陶宛的各个地方捕获了小型啮齿动物（n=139），这些地方以前在探查蜱中检测到了TBEV。用每种啮齿动物样品接种鼠神经母细胞瘤神经-2a细胞以最大化检测啮齿动物样品中的病毒RNA的机会。在74.8%（CI95%66.7-81.1）的脑和/或内脏器官混合悬液中检测到TBEVRNA，在Neuro-2a细胞中进行样品培养后，患病率显着增加。此外，在啮齿动物悬浮液的细胞培养分离物中，啮齿动物诱捕的平均每月空气温度与TBEVRNA患病率之间存在很强的相关性（r=0.88;p<0.05）。在细胞培养前PCR阴性。这项研究表明，野生啮齿动物是确定TBEV病灶的合适前哨动物。此外,研究结果表明，细胞培养中的样品培养是一种将TBEV病毒载量增加到可检测量的高效方法。
