PDF(Pubmed)
Abstract：
This study focuses on optimizing the mutagenesis process for Morchella eximia (Mel-7) mycelia through atmospheric and room temperature plasma (ARTP) mutation and explores the resultant thermal adaptability and physiological responses of mutant strains. This research demonstrated a clear relationship between ARTP mutagenesis exposure duration and lethality rate, indicating that an exposure time of 40 s resulted in the optimal balance of inducing mutations without causing excessive mortality. Additionally, this study established 43 °C as the ideal screening temperature for identifying mutant strains with enhanced heat resistance, as this temperature significantly challenges the mycelia while allowing thermotolerant strains to be distinguishable. Among the screened mutants, strains L21, L23, L44, and L47 exhibited superior growth and high-temperature tolerance, with notable resilience at 30 °C, highlighting their enhanced adaptability to above-optimal temperatures. Furthermore, this research delved into biochemical responses, including lipid peroxidation and non-enzymatic antioxidant content, highlighting the diverse mechanisms, such as enhanced lipid peroxidation resistance and increased antioxidant content, employed by mutant strains to adapt to temperature fluctuations. The activities of antioxidant enzymes, including peroxidase (POD) and superoxide dismutase (SOD), were shown to be significantly influenced by temperature elevations, illustrating their critical roles in the thermal adaptation of mutant strains. These findings shed light on the importance of considering mutation duration and temperature screening in the development of thermotolerant fungal strains with potential applications in various industries. This study\'s breakthrough lies in its comprehensive understanding of the thermal adaptability of Mel-7 mycelia and the identification of promising mutant strains, offering valuable insights for both academic and industrial purposes.
摘要：
本研究通过大气和室温等离子体（ARTP）突变优化了羊肚菌（Mel-7）菌丝体的诱变过程，并探讨了突变菌株的热适应性和生理反应。这项研究证明了ARTP诱变暴露持续时间和致死率之间的明确关系，表明40s的暴露时间导致诱导突变的最佳平衡，而不会导致过度死亡。此外,本研究将43°C作为鉴定耐热性增强的突变菌株的理想筛选温度，因为这个温度显着挑战菌丝体，同时允许耐热菌株是可区分的。在筛选的突变体中，菌株L21、L23、L44和L47表现出优异的生长和高温耐受性，在30°C时具有显著的弹性，突出了它们对高于最佳温度的适应性。此外，这项研究深入研究了生化反应，包括脂质过氧化和非酶抗氧化剂含量，突出多样化的机制，如增强的抗脂质过氧化能力和增加的抗氧化剂含量，被突变菌株用来适应温度波动。抗氧化酶的活性，包括过氧化物酶（POD）和超氧化物歧化酶（SOD），被证明受温度升高的影响很大，说明了它们在突变菌株的热适应中的关键作用。这些发现揭示了在开发具有潜在应用于各种行业的耐热性真菌菌株中考虑突变持续时间和温度筛选的重要性。本研究的突破在于全面了解了Mel-7菌丝体的热适应性,为学术和工业目的提供有价值的见解。
