PDF(Pubmed)
Abstract：
Antimicrobial resistance (AMR) is a global public health threat. Quality data are needed to address the rise of multidrug-resistant clones, particularly in sub-Saharan Africa. In this study, we analysed the prevalence, antimicrobial resistance profile, and presence of genes encoding extended-spectrum β-lactamase-producing Escherichia coli (ESBL-Ec) and Klebsiella pneumoniae (ESBL-Kp) in environmental samples from Ouagadougou, Burkina Faso. Of 264 samples collected, 95 (36%) and 74 (28%) contained ESBL-Kp and ESBL-Ec, respectively. ESBL-Kp was more prevalent in runoff water and in treated and untreated wastewater, while ESBL-Ec was more prevalent in manure. Interestingly, wastewater treatment did not significantly reduce the recovery of ESBL bacteria. As expected, resistance to third- and fourth-generation cephalosporins was predominant, and rare for second generation cefoxitin. Interestingly, all the isolates from treated wastewater were susceptible to ampicillin and piperacillin, while all the other clones were resistant to these antibiotics. Regarding the ESBL-encoding genes, the blaCTX-M family was the most abundant, with the blaCTX-M1 subfamily being the most prevalent. Carriage of combinations of ESBL genes was common, with the majority of the isolates harbouring 2-4 different genes. This study highlights the need for active surveillance to manage the risk of exposure to ESBL bacteria in Burkina Faso.
摘要：
抗菌素耐药性（AMR）是全球公共卫生威胁。需要高质量的数据来解决多药耐药克隆的兴起，特别是在撒哈拉以南非洲。在这项研究中,我们分析了患病率，抗菌素耐药性概况，以及在来自瓦加杜古的环境样品中存在编码产超广谱β-内酰胺酶的大肠杆菌（ESBL-Ec）和肺炎克雷伯菌（ESBL-Kp）的基因，布基纳法索。在收集的264个样本中，95(36%)和74(28%)含有ESBL-Kp和ESBL-Ec，分别。ESBL-Kp在径流水以及经处理和未经处理的废水中更为普遍，而ESBL-Ec在粪肥中更为普遍。有趣的是,废水处理并没有显著降低ESBL细菌的回收率。不出所料,对第三代和第四代头孢菌素的耐药性占主导地位，第二代头孢西丁很少见。有趣的是,处理过的废水中的所有分离物均对氨苄西林和哌拉西林敏感，而所有其他克隆对这些抗生素都有抗性。关于ESBL编码基因，blaCTX-M家族是最丰富的,blaCTX-M1亚家族是最普遍的。携带ESBL基因的组合是常见的，大多数分离株携带2-4种不同的基因。这项研究强调了在布基纳法索进行主动监测以管理暴露于ESBL细菌的风险的必要性。
