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Abstract：
This study investigates the roles of mucosal-associated invariant T (MAIT) cells and Vα7.2+/CD161- T cells in skin diseases, focusing on atopic dermatitis. MAIT cells, crucial for bridging innate and adaptive immunity, were analyzed alongside Vα7.2+/CD161- T cells in peripheral blood samples from 14 atopic dermatitis patients and 10 healthy controls. Flow cytometry and machine learning algorithms were employed for a comprehensive analysis. The results indicate a significant decrease in MAIT cells and CD69 subsets in atopic dermatitis, coupled with elevated CD38 and polyfunctional MAIT cells producing TNFα and Granzyme B (TNFα+/GzB+). Vα7.2+/CD161- T cells in atopic dermatitis exhibited a decrease in CD8 and IFNγ-producing subsets but an increase in CD38 activated and IL-22-producing subsets. These results highlight the distinctive features of MAIT cells and Vα7.2+/CD161- T cells and their different roles in the pathogenesis of atopic dermatitis and provide insights into their potential roles in immune-mediated skin diseases.
摘要：
本研究探讨了粘膜相关不变T（MAIT）细胞和Vα7.2+/CD161-T细胞在皮肤病中的作用，专注于特应性皮炎。MAIT细胞,对于桥接先天免疫和适应性免疫至关重要，与来自14名特应性皮炎患者和10名健康对照的外周血样本中的Vα7.2+/CD161-T细胞一起分析。采用流式细胞术和机器学习算法进行综合分析。结果表明，特应性皮炎中MAIT细胞和CD69亚群的显着减少，结合升高的CD38和产生TNFα和颗粒酶B（TNFα/GzB）的多功能MAIT细胞。特应性皮炎中的Vα7.2/CD161-T细胞显示CD8和IFNγ产生亚群减少，但CD38激活和IL-22产生亚群增加。这些结果突出了MAIT细胞和Vα7.2+/CD161-T细胞的独特特征及其在特应性皮炎发病机理中的不同作用，并为其在免疫介导的皮肤病中的潜在作用提供了见解。
