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Abstract：
Sweetpotato (Ipomoea batatas L.) is a strategic crop with both economic and energy value. However, improving sweetpotato varieties through traditional breeding approaches can be a time-consuming and labor-intensive process due to the complex genetic nature of sweetpotato as a hexaploid species (2n = 6x = 90). Double haploid (DH) breeding, based on in vivo haploid induction, provides a new approach for rapid breeding of crops. The success of haploid induction can be achieved by manipulating specific genes. Two of the most critical genes, DMP (DUF679 membrane proteins) and MTL (MATRILINEAL), have been shown to induce haploid production in several species. Here, we identified and characterized DMP and MTL genes in sweetpotato using gene family analysis. In this study, we identified 5 IbDMPs and 25 IbpPLAs. IbDMP5 and IbPLAIIs (IbPLAIIκ, IbPLAIIλ, and IbPLAIIμ) were identified as potential haploid induction (HI) genes in sweetpotato. These results provide valuable information for the identification and potential function of HI genes in sweetpotato and provide ideas for the breeding of DH lines.
摘要：
甘薯（IpomoeabatatasL.）是一种具有经济和能源价值的战略作物。然而,由于甘薯作为六倍体物种的复杂遗传性质（2n=6x=90），通过传统育种方法改进甘薯品种可能是一个耗时耗力的过程。双单倍体(DH)育种,基于体内单倍体诱导，为作物快速育种提供了新的途径。单倍体诱导的成功可以通过操纵特定基因来实现。两个最关键的基因,DMP（DUF679膜蛋白）和MTL（MATRILINEAL），已被证明在几个物种中诱导单倍体产生。这里,我们使用基因家族分析鉴定并表征了甘薯中的DMP和MTL基因。在这项研究中,我们确定了5个IbDMPs和25个IbPPLA。IbDMP5和IbPLAIs(IbPLAIIκ,IbPLAIIλ,和IbPLAIIμ）被鉴定为甘薯中的潜在单倍体诱导（HI）基因。这些结果为甘薯HI基因的鉴定和潜在功能提供了有价值的信息，并为DH品系的选育提供了思路。
