PDF(Pubmed)
Abstract：
Magnolia kwangsiensis, a dioecious tree native to China, is recognized not only for its status as an at-risk species but also for its potential in therapeutic applications courtesy of its bioactive compounds. However, the genetic underpinnings of its leaf development and compound biosynthesis are not well documented. Our study aims to bridge this knowledge gap through comparative transcriptomics, analyzing gene expression through different leaf maturation stages. We studied the transcriptome of M. kwangsiensis leaves by applying RNA sequencing at juvenile, tender, and mature phases. We identified differentially expressed genes (DEGs) to explore transcriptional changes accompanying the developmental trajectory. Our analysis delineates the transcriptional landscape of over 20,000 genes with over 6000 DEGs highlighting significant transcriptional shifts throughout leaf maturation. Mature leaves demonstrated upregulation in pathways related to photosynthesis, cell wall formation, and polysaccharide production, affirming their structural integrity and specialized metabolic functions. Our GO and KEGG enrichment analyses underpin these findings. Furthermore, we unveiled coordinated gene activity correlating development with synthesizing therapeutically relevant polysaccharides. We identified four novel glycosyltransferases potentially pivotal in this synergistic mechanism. Our study uncovers the complementary evolutionary forces that concurrently sculpt structural and chemical defenses. These genetic mechanisms calibrate leaf tissue resilience and biochemical efficacy.
摘要：
广玉兰,一种原产于中国的雌雄异株树，不仅因为其作为危险物种的地位，而且因为其生物活性化合物在治疗应用中的潜力而得到认可。然而,其叶片发育和化合物生物合成的遗传基础没有得到很好的记录。我们的研究旨在通过比较转录组学弥合这一知识差距，分析不同叶片成熟期的基因表达。我们通过在幼体上应用RNA测序研究了广山叶片的转录组，tender,成熟的阶段。我们确定了差异表达基因（DEGs）以探索伴随发育轨迹的转录变化。我们的分析描绘了超过20,000个基因的转录景观，超过6000个DEGs突出了整个叶片成熟的显着转录变化。成熟的叶子在与光合作用相关的途径中表现出上调，细胞壁形成,和多糖生产，确认其结构完整性和专门的代谢功能。我们的GO和KEGG富集分析支持了这些发现。此外，我们揭示了协调的基因活性，将发育与合成治疗相关的多糖相关联。我们鉴定了四种在这种协同机制中潜在关键的新型糖基转移酶。我们的研究揭示了同时塑造结构和化学防御的互补进化力量。这些遗传机制校准叶片组织恢复力和生化功效。
