PDF(Pubmed)
Abstract：
For marine invertebrates, the disruption of organismal physiology and behavior by nanoplastics (NPs) has been extensively reported. Heat shock proteins (Hsps) are important for redundant protein breakdown, environmental changes, and intracellular protein transport. An exhaustive identification of Hsp70 genes and an experiment where different concentrations of NPs were stressed were performed to study how Hsp70 genes respond to NPs stress in Monodonta labio. Our results identified 15 members of Hsp70 within the genome of M. labio and provided insights into their responses to different concentrations of acute NP stress. Phylogenetic analyses revealed extensive amplification of the Hsp70 genes from the Hsc70 subfamily, with gene duplication events. As a result of NP stress, five of fifteen genes showed significant upregulation or downregulation. Three Hsp70 genes were highly expressed at an NP concentration of 0.1 mg/L, and no genes were downregulated. At 10 mg/L, they showed significant upregulation of two genes and significant downregulation of two genes. At 1 mg/L treatment, three genes were significantly downregulated, and no genes were significantly upregulated. Moreover, a purifying selection was revealed using a selection test conducted on duplicate gene pairs, indicating functional redundancy. This work is the first thorough examination of the Hsp70s in Archaeogastropoda. The findings improve knowledge of Hsp70s in molluscan adaptation to NP stress and intertidal living and offer essential data for the biological study of M. labio.
摘要：
对于海洋无脊椎动物，已广泛报道了纳米塑料（NPs）对生物体生理和行为的破坏。热休克蛋白（Hsps）对于冗余蛋白质分解很重要，环境变化,和细胞内蛋白质转运。进行了Hsp70基因的详尽鉴定和对不同浓度的NP进行应激的实验，以研究Hsp70基因如何对Monodontalabio中的NP应激做出反应。我们的结果在拉比奥的基因组中鉴定了15个Hsp70成员，并提供了他们对不同浓度的急性NP应激的反应的见解。系统发育分析揭示了来自Hsc70亚家族的Hsp70基因的广泛扩增，基因复制事件。由于NP压力，15个基因中有5个显示出显着的上调或下调。3个Hsp70基因在NP浓度为0.1mg/L时高表达,没有基因下调。在10毫克/升，它们显示了两个基因的显着上调和两个基因的显着下调。在1mg/L处理，三个基因显著下调，没有基因显著上调。此外，使用对重复基因对进行的选择测试揭示了纯化选择，指示功能冗余。这项工作是对古氏腹足动物Hsp70的首次彻底检查。研究结果提高了软体动物对NP压力和潮间带生活的适应中Hsp70的知识，并为M.labio的生物学研究提供了必要的数据。
