PDF(Pubmed)
Abstract：
Lidocaine, a local anesthetic widely used in dentistry, is esteemed for its efficacy and safety. Recent research reveals its additional role in modulating the immune system, and particularly in reducing inflammation crucial for protecting tooth-supporting tissues. Notably, monocytes and macrophages, essential cellular components overseeing various physiological and pathological processes, stand as potential mediators of lidocaine\'s effects. Therefore, this study aimed to investigate how lidocaine influences cell behavior using RNA sequencing. To investigate the effect of lidocaine on THP-1 cells\' behavior, we performed an MTT assay and RNA-Seq along with qPCR analyses to evaluate the transcriptomic and proteomic changes in THP-1 cells. Our results showed that a high dose of lidocaine (>1 mM) had a significant cytotoxic effect on THP-1 cells. However, a lidocaine dose lower than 0.5 mM induced a mixed anti-inflammatory profile by significantly upregulating tissue remodeling (GDF15, FGF7, HGF, COL4A3, COL8A2, LAMB2, LAMC2, PDGFRA, and VEGFA) and through the resolution of inflammation (Cpeb4, Socs1, Socs2, Socs3, Dusp1, Tnfaip3, and Gata3) gene cassettes. This study explores the effect of lidocaine on the THP-1 in the M2-like healing phenotype and provides potential applications of lidocaine\'s therapeutic effectiveness in dental tissue repair.
摘要：
利多卡因,一种广泛用于牙科的局部麻醉剂，因其功效和安全性而受到尊重。最近的研究揭示了它在调节免疫系统中的额外作用，尤其是减少炎症对保护牙齿支撑组织至关重要。值得注意的是,单核细胞和巨噬细胞，监督各种生理和病理过程的基本细胞成分，作为利多卡因作用的潜在介质。因此,本研究旨在利用RNA测序研究利多卡因如何影响细胞行为.为了研究利多卡因对THP-1细胞行为的影响，我们进行了MTT分析和RNA-Seq以及qPCR分析，以评估THP-1细胞的转录组和蛋白质组变化。我们的结果表明，高剂量的利多卡因（>1mM）对THP-1细胞具有显着的细胞毒性作用。然而,低于0.5mM的利多卡因剂量通过显著上调组织重塑(GDF15，FGF7，HGF，COL4A3,COL8A2,LAMB2,LAMC2,PDGFRA,和VEGFA）并通过炎症（Cpeb4，Socs1，Socs2，Socs3，Dusp1，Tnfaip3和Gata3）基因盒的解决。本研究探讨了利多卡因对M2样愈合表型中THP-1的影响，并提供了利多卡因在牙齿组织修复中的潜在应用。
