PDF(Pubmed)
Abstract：
Saffron (Crocus sativus L.) is one of the most expensive spices in the world, boasting rich medicinal and edible value. However, the effective development of active natural substances in saffron is still limited. Currently, there is a lack of comprehensive studies on the saffron stigma protein, and the main effect peptides have not been identified. In this study, the total protein composition of saffron stigmas was analyzed, and two main antioxidant peptides (DGGSDYLGK and VDPYFNK) were identified, which showed high antioxidant activity. Then, the stability of two peptides was further evaluated. Furthermore, our results suggested that these two peptides may protect HepG2 cells from H2O2-induced oxidative damage by significantly improving the activity of endogenous antioxidant enzymes and reducing the malondialdehyde (MDA) content. Collectively, we identified two peptides screened from the saffron protein possessing good antioxidant activity and stability, making them promising candidates for use as functional foods, etc., for health promotion. Our findings indicated that proteomic analysis together with peptide identification is a good method for exploitation and utilization of spice plants.
摘要：
藏红花（CrocussativusL.）是世界上最昂贵的香料之一，具有丰富的药用和食用价值。然而,藏红花中活性天然物质的有效开发仍然有限。目前,缺乏对藏红花柱头蛋白的全面研究，和主要的作用肽尚未确定。在这项研究中,分析了藏红花柱头的总蛋白质组成，并鉴定了两种主要的抗氧化肽（DGGSDYLGK和VDPYFNK），表现出较高的抗氧化活性。然后，进一步评价两种肽的稳定性。此外，我们的结果表明，这两种肽可能通过显着提高内源性抗氧化酶的活性和降低丙二醛（MDA）含量来保护HepG2细胞免受H2O2诱导的氧化损伤。总的来说，我们从藏红花蛋白中筛选出两种具有良好抗氧化活性和稳定性的多肽，使它们有希望用作功能性食品，等。,促进健康。我们的发现表明，蛋白质组学分析和肽鉴定是开发和利用香料植物的良好方法。
