PDF(Pubmed)
Abstract：
The cultivation of Crocus sativus L. to obtain the saffron spice generates a large amount of biowaste, constituted mainly by the flower\'s tepals. The aim of this work was to evaluate the antioxidant and dermo-protective effect of a complex methanolic extract of C. sativus tepals. The extract\'s major phenolic content was analyzed using ultra-high performance liquid chromatography with electrospray ionization, coupled with quadrupole-time-of-flight-mass spectrometry (UHPLC-ESI-QTOF-MS). Then, the antioxidant in vitro activity of the extract was studied and related to their chemical composition. Likewise, the effect on intracellular ROS levels in HepG2 and Hs27 cell culture was determined in normal culture and under hydrogen-peroxide-induced oxidative stress. Finally, tyrosinase, hyaluronidase, collagenase, elastase, and xanthine oxidase assays were carried out to determine the dermo-protective capacity of the extract. The high polyphenol content, including flavonoids and anthocyanins, explains the antioxidant effect of the extract both in vitro and in culture assays. The extract has a significant and remarkable protective capacity against oxidative stress induced in culture of the two studied cell lines. It is also remarkable in its ability to inhibit hyaluronidase, tyrosinase, and xanthine oxidase. Results pointed out this biowaste extract as a promising ingredient in the composition of cosmetics.
摘要：
种植藏红花香料会产生大量的生物废料，主要由花的花被构成。这项工作的目的是评估C.sativustepals复合甲醇提取物的抗氧化和皮肤保护作用。提取物的主要酚类含量分析使用超高效液相色谱法与电喷雾电离，结合四极杆-飞行时间-质谱(UHPLC-ESI-QTOF-MS)。然后，研究了提取物的体外抗氧化活性，并与其化学组成有关。同样，在正常培养和过氧化氢诱导的氧化应激下，确定了对HepG2和Hs27细胞培养物中细胞内ROS水平的影响。最后,酪氨酸酶,透明质酸酶，胶原酶,弹性蛋白酶,进行黄嘌呤氧化酶测定以确定提取物的皮肤保护能力。多酚含量高，包括类黄酮和花青素,解释了提取物在体外和培养试验中的抗氧化作用。该提取物对两种研究细胞系的培养物中诱导的氧化应激具有显着和显着的保护能力。它还具有显著的抑制透明质酸酶的能力，酪氨酸酶,和黄嘌呤氧化酶.结果表明，这种生物酱提取物是化妆品组合物中有前途的成分。
