Abstract:
This research evaluated the impacts of selenomethionine (Se-Met) on hepatic functions, oxidative stress, mitochondrial function, and apoptosis of piglets fed deoxynivalenol (DON)-contaminated diets. Twenty-four piglets were allocated four dietary treatments (n = 6) in a 28-day feeding trial. The four treatments included the control group, which received 0.3 mg/kg of Se (as Se-Met) without DON treatment, and the DON treatment groups received 0, 0.3, or 0.5 mg/kg Se as Se-Met. A dietary addition of 0.5 mg/kg Se improved liver pathology and reduced serum aspartate aminotransferase and lactate dehydrogenase levels in piglets fed DON-contaminated diets. Furthermore, 0.5 mg/kg Se mitigated the oxidative stress and apoptosis of piglets fed DON-contaminated diets, as indicated by the decreased reactive oxygen species level, and the down-regulated mRNA levels of NRF-1, Bax, and CASP9 in the liver. Importantly, 0.5 mg/kg Se enhanced the hepatic antioxidant capacity, as evidenced by increased hepatic total antioxidant capacity, catalase, glutathione peroxidase, and total superoxide dismutase activities, as well as the up-regulated mRNA levels of Nrf2, Gclm, NQO1, SOD1, and GPX1 in the liver. Moreover, 0.5 mg/kg Se down-regulated the p-JNK protein level in the liver of piglets fed DON-contaminated diets. Collectively, Se-Met supplementation mitigated liver dysfunction, oxidative injury, and apoptosis through enhancing antioxidant capacity and inhibiting the JNK MAPK pathway in piglets fed DON-contaminated diets.
摘要:
这项研究评估了硒代蛋氨酸(Se-Met)对肝功能的影响,氧化应激,线粒体功能,饲喂脱氧雪腐镰刀菌烯醇(DON)污染日粮的仔猪和细胞凋亡。在28天的喂养试验中,为24只仔猪分配了四种饮食处理(n=6)。四种治疗方法包括对照组,接受0.3mg/kgSe(作为Se-Met),未经DON处理,DON治疗组接受0、0.3或0.5mg/kgSe作为Se-Met。日粮添加0.5mg/kg硒可改善DON污染日粮仔猪的肝脏病理,并降低血清天冬氨酸转氨酶和乳酸脱氢酶水平。此外,0.5mg/kg硒可减轻DON污染日粮仔猪的氧化应激和细胞凋亡,正如活性氧水平降低所表明的那样,和下调的NRF-1,Bax,和肝脏中的CASP9。重要的是,0.5mg/kg硒提高肝脏的抗氧化能力,肝脏总抗氧化能力增加,过氧化氢酶,谷胱甘肽过氧化物酶,和总超氧化物歧化酶活性,以及Nrf2,Gclm的mRNA水平上调,NQO1、SOD1和GPX1在肝脏中。此外,0.5mg/kgSe下调了饲喂DON污染日粮的仔猪肝脏中p-JNK蛋白的水平。总的来说,Se-Met补充剂减轻肝功能障碍,氧化损伤,通过增强DON污染日粮仔猪的抗氧化能力和抑制JNKMAPK通路实现细胞凋亡。