Abstract：
UNASSIGNED: The objective of this study was to investigate the glycolytic activity of adenomyosis, which is characterized by malignant biological behaviors including abnormal cell proliferation, migration, invasion, cell regulation, and epithelial-mesenchymal transition.
UNASSIGNED: From January 2021 to August 2022, a total of 15 patients who underwent total hysterectomy for adenomyosis and 14 patients who had non-endometrial diseases, specifically with cervical squamous intraepithelial neoplasia and uterine myoma, were included in this study. Myometrium with ectopic endometrium from patients with adenomyosis while normal myometrium from patients in the control group were collected. All samples were confirmed by a histopathological examination. The samples were analyzed by liquid chromatography-mass spectrometry (LC-MS), real-time quantitative PCR, NAD+/NADH assay kit as well as the glucose and lactate assay kits.
UNASSIGNED: Endometrial stroma and glands could be observed within the myometrium of patients in the adenomyosis group. We found that the mRNA expressions of HK1, PFKFB3, glyceraldehyde-3-phospate dehydrogenase (GAPDH), PKM2, and PDHA as well as the protein expressions of PFKFB3 were elevated in ectopic endometrial tissues of the adenomyosis group as compared to normal myometrium of the control group. The level of fructose 1,6-diphosphate was increased while NAD + and NAD+/NADH ratio were decreased compared with the control group. Besides, increased glucose consumption and lactate production were observed in myometrium with ectopic endometrium.
UNASSIGNED: We concluded that altered glycolytic phenotype of the myometrium with ectopic endometrium in women with adenomyosis may contribute the development of adenomyosis.
UNASSIGNED: From January 2021 to August 2022, a total of 15 patients who underwent total hysterectomy for adenomyosis and 14 patients who had non-endometrial diseases, specifically with cervical squamous intraepithelial neoplasia and uterine myoma, were included in this study. Myometrium with ectopic endometrium from patients with adenomyosis while normal myometrium from patients in the control group were collected. All samples were confirmed by a histopathological examination. The samples were analyzed by liquid chromatography-mass spectrometry (LC-MS), real-time quantitative PCR, NAD+/NADH assay kit as well as the glucose and lactate assay kits.
UNASSIGNED: Endometrial stroma and glands could be observed within the myometrium of patients in the adenomyosis group. We found that the mRNA expressions of HK1, PFKFB3, glyceraldehyde-3-phospate dehydrogenase (GAPDH), PKM2, and PDHA as well as the protein expressions of PFKFB3 were elevated in ectopic endometrial tissues of the adenomyosis group as compared to normal myometrium of the control group. The level of fructose 1,6-diphosphate was increased while NAD + and NAD+/NADH ratio were decreased compared with the control group. Besides, increased glucose consumption and lactate production were observed in myometrium with ectopic endometrium.
UNASSIGNED: We concluded that altered glycolytic phenotype of the myometrium with ectopic endometrium in women with adenomyosis may contribute the development of adenomyosis.
摘要：
■这项研究的目的是研究子宫腺肌病的糖酵解活性，其特征是恶性生物学行为，包括异常的细胞增殖，迁移,入侵,细胞调节，和上皮-间质转化。
■从2021年1月到2022年8月，共有15例因子宫腺肌病接受全子宫切除术的患者和14例非子宫内膜疾病的患者，特别是宫颈鳞状上皮内瘤变和子宫肌瘤，包括在这项研究中。收集子宫腺肌病患者子宫内膜异位的子宫肌层，而对照组患者正常子宫肌层。所有样品均通过组织病理学检查确认。通过液相色谱-质谱(LC-MS)分析样品,实时定量PCR,NAD+/NADH测定试剂盒以及葡萄糖和乳酸测定试剂盒。
■子宫腺肌病组患者的子宫肌层内可观察到子宫内膜间质和腺体。我们发现HK1，PFKFB3，甘油醛-3-磷酸脱氢酶（GAPDH）的mRNA表达，与对照组的正常子宫肌层相比，子宫腺肌病组的异位子宫内膜组织中PKM2和PDHA以及PFKFB3的蛋白表达升高。与对照组相比，1,6-二磷酸果糖水平升高，而NAD和NAD/NADH比值降低。此外,在子宫内膜异位的子宫肌层中观察到葡萄糖消耗和乳酸产生增加。
■我们得出结论，子宫腺肌病女性子宫内膜异位的子宫肌层糖酵解表型的改变可能有助于子宫腺肌病的发展。
■从2021年1月到2022年8月，共有15例因子宫腺肌病接受全子宫切除术的患者和14例非子宫内膜疾病的患者，特别是宫颈鳞状上皮内瘤变和子宫肌瘤，包括在这项研究中。收集子宫腺肌病患者子宫内膜异位的子宫肌层，而对照组患者正常子宫肌层。所有样品均通过组织病理学检查确认。通过液相色谱-质谱(LC-MS)分析样品,实时定量PCR,NAD+/NADH测定试剂盒以及葡萄糖和乳酸测定试剂盒。
■子宫腺肌病组患者的子宫肌层内可观察到子宫内膜间质和腺体。我们发现HK1，PFKFB3，甘油醛-3-磷酸脱氢酶（GAPDH）的mRNA表达，与对照组的正常子宫肌层相比，子宫腺肌病组的异位子宫内膜组织中PKM2和PDHA以及PFKFB3的蛋白表达升高。与对照组相比，1,6-二磷酸果糖水平升高，而NAD和NAD/NADH比值降低。此外,在子宫内膜异位的子宫肌层中观察到葡萄糖消耗和乳酸产生增加。
■我们得出结论，子宫腺肌病女性子宫内膜异位的子宫肌层糖酵解表型的改变可能有助于子宫腺肌病的发展。
