OBJECTIVE: To evaluate the anti-biofilm effectiveness of denture cleaning protocols in hospitalized patients.
METHODS: The maxillary complete dentures (MCDs) of 340 hospitalized participants were randomly cleaned once using one of the following 17 protocols (n=20): brushing with distilled water, toothpaste, or neutral liquid soap (controls); immersion in chemical solutions (1% sodium hypochlorite, alkaline peroxide, 0.12% or 2% chlorhexidine digluconate), or microwave irradiation (650 W for 3 min) combined or not with brushing. Before and after the application of the protocols, the biofilm of the intaglio surface of the MCDs was evaluated using two methods: denture biofilm coverage area (%) and microbiological quantitative cultures on blood agar and Sabouraud Dextrose Agar (CFU/mL). Data were subjected to the Wilcoxon and Kruskal-Wallis tests (α=0.05).
RESULTS: All 17 protocols significantly reduced the percentage area of denture biofilm and microbial and fungal load (P<0.05). The highest percentage reductions in the area of denture biofilm were observed for 1% hypochlorite solution with or without brushing and for 2% chlorhexidine solution and microwave irradiation only in association with brushing (P<0.05). The greatest reductions in microbial and fungal load were found for the groups that used solutions of 2% chlorhexidine and 1% hypochlorite and microwave irradiation, regardless of the association with brushing (P<0.05).
CONCLUSIONS: A single immersion for 10 min in 1% sodium hypochlorite, even in the absence of brushing, proved to be a straightforward, rapid, low-cost, and effective protocol for cleaning the dentures of hospitalized patients.
目的:评估住院患者义齿清洁方案的抗生物膜效果。
方法:使用以下17种方案之一(n=20)随机清洁340名住院参与者的上颌全口义齿(MCD):用蒸馏水刷牙,牙膏,或中性液体肥皂(对照);浸入化学溶液(1%次氯酸钠,碱性过氧化物,0.12%或2%二葡萄糖酸氯己定),或微波照射(650W3分钟)结合或不刷牙。在应用协议之前和之后,使用两种方法评估MCD的凹版表面的生物膜:义齿生物膜覆盖面积(%)和在血琼脂和Sabouraud葡萄糖琼脂(CFU/mL)上的微生物定量培养物。对数据进行Wilcoxon和Kruskal-Wallis检验(α=0.05)。
结果:所有17种方案均显着降低了义齿生物膜的面积百分比以及微生物和真菌负荷(P<0.05)。对于有或没有刷牙的1%次氯酸盐溶液以及仅与刷牙相关的2%氯己定溶液和微波照射,观察到义齿生物膜面积的最高百分比减少(P<0.05)。对于使用2%氯己定和1%次氯酸盐溶液和微波辐射的组,发现微生物和真菌负荷的最大减少。与刷牙无关(P<0.05)。
结论:在1%次氯酸钠中浸泡10分钟,即使在没有刷牙的情况下,被证明是直截了当的,快速,低成本,以及清洁住院患者假牙的有效协议。