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Abstract：
Magnetic molecularly imprinted polymers (MMIPs) have fused molecular imprinting technology with magnetic separation technology, emerging as an innovative material capable of recognizing specific molecules and efficiently separating target substances. Their application to the extraction and purification of mycotoxins has great potential, due to the toxicity and economic impact of these contaminants. In this work, MMIP has been proposed as a sample treatment for the determination of main four aflatoxins (B1, B2, G1 and G2) in pig feed. The MMIP was formed through the integration of magnetic material (Fe3O4) with commercial molecularly imprinted polymers, avoiding the synthesis step and, therefore, simplifying the process. The analyses were carried out by high-performance liquid chromatography with fluorescence detection and the method was validated and limits of quantification (LOQs) between 0.09 and 0.47 ng/g were obtained, below the allowed or recommended levels by the European Union. Repeatability and intermediate precision showed relative standard deviations lower than 10% in all cases and trueness ranged from 92 to 111%. Finally, the proposed method was applied to 31 real pig feed samples, detecting aflatoxins with concentrations between 0.2 and 3.2 ng/g.
摘要：
磁性分子印迹聚合物(MMIP)具有融合分子印迹技术与磁分离技术,成为一种能够识别特定分子并有效分离目标物质的创新材料。它们应用于霉菌毒素的提取和纯化具有很大的潜力,由于这些污染物的毒性和经济影响。在这项工作中,MMIP已被建议作为样品处理，用于测定猪饲料中的主要四种黄曲霉毒素（B1，B2，G1和G2）。MMIP是通过将磁性材料（Fe3O4）与商业分子印迹聚合物集成而形成的，避免合成步骤，因此,简化过程。通过高效液相色谱和荧光检测进行分析，并对该方法进行了验证，获得了0.09至0.47ng/g的定量限（LOQs）。低于欧盟允许或建议的水平。在所有情况下，重复性和中等精度显示相对标准偏差均低于10％，真实性范围为92％至111％。最后,将所提出的方法应用于31个真实的猪饲料样品，检测浓度在0.2和3.2ng/g之间的黄曲霉毒素。
