PDF(Pubmed)
Abstract：
Campylobacter species, especially C. jejuni and C. coli, are the main zoonotic bacteria causing human gastroenteritis. A variety of Campylobacter species has been reported in wild birds, posing a potential avian-human transmission pathway. Currently, there has been little surveillance data on Campylobacter carriage in migratory birds in China. In the current work, fresh fecal droppings from individual migratory birds were collected at four bird wintering/stopover sites in China from May 2020 to March 2021. Nucleic acid was extracted and tested for Campylobacter with PCR-based methods. Overall, 73.8% (329/446) of the samples were positive for Campylobacter, demonstrating location and bird host specificity. Further speciation revealed the presence of C. jejuni, C. coli, C. lari, C. volucris, and an uncharacterized species, which all harbored a variety of virulence factors. Phylogenetic analysis performed on concatenated 16S rRNA-atpA-groEL genes elucidated their genetic relationship, demonstrating both inter- and intra-species diversity. The wide distribution and high diversity of Campylobacter spp. detected in migratory birds in China indicated potential transmission across territories. The existence of virulence factors in all of these species highlighted their public health importance and the necessity of monitoring and controlling Campylobacter and other pathogens carried by migratory birds.
摘要：
弯曲杆菌属,尤其是空肠杆菌和大肠杆菌,是引起人类胃肠炎的主要人畜共患病细菌。据报道，野生鸟类中存在多种弯曲杆菌，构成了潜在的禽人传播途径。目前,在中国，很少有关于候鸟弯曲杆菌携带的监测数据。在目前的工作中,从2020年5月至2021年3月，在中国的四个鸟类越冬/中途停留地点收集了个别候鸟的新鲜粪便。提取核酸并用基于PCR的方法测试弯曲杆菌。总的来说,73.8%(329/446)的样本为弯曲杆菌阳性,展示位置和鸟类宿主特异性。进一步的物种形成揭示了空肠弯曲杆菌的存在，大肠杆菌，C.拉里,C.volucris,和一个没有特征的物种，都有多种毒力因子.对连接的16SrRNA-atpA-groEL基因进行的系统发育分析阐明了它们的遗传关系，证明了物种间和物种内的多样性。弯曲菌分布广泛，多样性高。在中国的候鸟中检测到，表明潜在的跨领土传播。所有这些物种中毒力因子的存在突显了它们的公共卫生重要性以及监测和控制候鸟携带的弯曲杆菌和其他病原体的必要性。
